Wemmer, Component Project Leader
David Wemmer is a Co-Principal Investigator who will oversee all
aspects of the execution of the proposed work in application of
NMR to protein screening and structure determination.
The NMR component of the project will apply multinuclear, multidimensional
solution methods to screen samples of expressed proteins for foldedness,
aggregation state, presence of flexible tails or domains, and to
determine structures of small proteins from the target organism,
Mycoplasma pneumoniae, or their homologs from other orgamisms.
Sets of experiments will be designed which are optimized for assignment
of resonances from proteins at different effective molecular weights.
Methods for automated analysis of data will be implemented and extended
to increase throughput. Structures will be interpreted to classify
fold, and to evaluate functional implications.