• Potential Target Homologues
• DNA Sequence
• Codon Usage
• Translation
• Amino Acid Sequence
• Protein Parameters
• Predicted properties
• Prediction details

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ATGAGTCATAAAGATTTTAATGGGCTTCAAGCCCCTCAGTTGTTAAGTTCGAGTTCCCCTGTTGCAAAGAAACAAAGTAG
TCATAAACTCAGGCATGCCTTAAAGCACGCCCGTTACTTAAACCATAGTAGTAAGCGTACCCTCAAGCATGCCTTAGAGC
TGCATGAAGACAACCAGGTCTTACTGGAAAAAGAAGGTAGTCCTAACTTCCAGGACTGGCTTTCTAAGCAACCTGGGGTC
AATAAAACCAGCCTTAAGTACAACAAAAGCTTGGGTAGTTGAATCTCTAAGGAAAGTAAACCGAAAAAGCGTTTTCCACC
GTACTTTACGTACAAGGGCAGTAAAACTACTCCAGAAGAGGCCAAAGCCTTGCAGCAAATGAAACAATCCCAAAAGCGCT
TCTTTCACGAAAATATGCACAGCTTTCTCAATGAAGTTGCACATAACCCAATGATCCAGCGCTTTAAGCAAAAGCAGGCC
AAACGGGCTGCTAATACGCGACAACGCACTTACAAATACCGTCAGTAA

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(176 codons)
fields: [triplet] [frequency: per thousand] ([number])

UUU34.1(   6)  UCU11.4(   2)  UAU0.0(   0)  UGU0.0(   0)  
UUC11.4(   2)  UCC11.4(   2)  UAC34.1(   6)  UGC0.0(   0)  
UUA28.4(   5)  UCA0.0(   0)  UAA5.7(   1)  UGA5.7(   1)  
UUG17.0(   3)  UCG5.7(   1)  UAG0.0(   0)  UGG5.7(   1)  

CUU17.0(   3)  CCU22.7(   4)  CAU39.8(   7)  CGU22.7(   4)  
CUC17.0(   3)  CCC0.0(   0)  CAC17.0(   3)  CGC17.0(   3)  
CUA0.0(   0)  CCA17.0(   3)  CAA45.5(   8)  CGA5.7(   1)  
CUG11.4(   2)  CCG11.4(   2)  CAG39.8(   7)  CGG5.7(   1)  

AUU0.0(   0)  ACU17.0(   3)  AAU28.4(   5)  AGU62.5(  11)  
AUC11.4(   2)  ACC11.4(   2)  AAC28.4(   5)  AGC17.0(   3)  
AUA0.0(   0)  ACA0.0(   0)  AAA73.9(  13)  AGA0.0(   0)  
AUG22.7(   4)  ACG11.4(   2)  AAG68.2(  12)  AGG5.7(   1)  

GUU11.4(   2)  GCU11.4(   2)  GAU5.7(   1)  GGU11.4(   2)  
GUC11.4(   2)  GCC39.8(   7)  GAC11.4(   2)  GGC5.7(   1)  
GUA0.0(   0)  GCA11.4(   2)  GAA39.8(   7)  GGA0.0(   0)  
GUG0.0(   0)  GCG0.0(   0)  GAG11.4(   2)  GGG11.4(   2)  

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atgagtcataaagattttaatgggcttcaagcccctcagttgttaagttcgagttcccct
 M  S  H  K  D  F  N  G  L  Q  A  P  Q  L  L  S  S  S  S  P 
gttgcaaagaaacaaagtagtcataaactcaggcatgccttaaagcacgcccgttactta
 V  A  K  K  Q  S  S  H  K  L  R  H  A  L  K  H  A  R  Y  L 
aaccatagtagtaagcgtaccctcaagcatgccttagagctgcatgaagacaaccaggtc
 N  H  S  S  K  R  T  L  K  H  A  L  E  L  H  E  D  N  Q  V 
ttactggaaaaagaaggtagtcctaacttccaggactggctttctaagcaacctggggtc
 L  L  E  K  E  G  S  P  N  F  Q  D  W  L  S  K  Q  P  G  V 
aataaaaccagccttaagtacaacaaaagcttgggtagttgaatctctaaggaaagtaaa
 N  K  T  S  L  K  Y  N  K  S  L  G  S  *  I  S  K  E  S  K 
ccgaaaaagcgttttccaccgtactttacgtacaagggcagtaaaactactccagaagag
 P  K  K  R  F  P  P  Y  F  T  Y  K  G  S  K  T  T  P  E  E 
gccaaagccttgcagcaaatgaaacaatcccaaaagcgcttctttcacgaaaatatgcac
 A  K  A  L  Q  Q  M  K  Q  S  Q  K  R  F  F  H  E  N  M  H 
agctttctcaatgaagttgcacataacccaatgatccagcgctttaagcaaaagcaggcc
 S  F  L  N  E  V  A  H  N  P  M  I  Q  R  F  K  Q  K  Q  A 
aaacgggctgctaatacgcgacaacgcacttacaaataccgtcagtaa
 K  R  A  A  N  T  R  Q  R  T  Y  K  Y  R  Q  * 

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MSHKDFNGLQAPQLLSSSSPVAKKQSSHKLRHALKHARYLNHSSKRTLKHALELHEDNQVLLEKEGSPNFQDWLSKQPGV
NKTSLKYNKSLGSWISKESKPKKRFPPYFTYKGSKTTPEEAKALQQMKQSQKRFFHENMHSFLNEVAHNPMIQRFKQKQA
KRAANTRQRTYKYRQ

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This information was obtained using the Protein Parameters tool on the ExPASy Molecular Biology Server.

Number of amino acids: 175

Molecular weight: 20516.4

Theoretical pI: 10.37

Amino acid composition:

Ala (A)  11	  6.3%
Arg (R)  10	  5.7%
Asn (N)  10	  5.7%
Asp (D)   3	  1.7%
Cys (C)   0	  0.0%
Gln (Q)  15	  8.6%
Glu (E)   9	  5.1%
Gly (G)   5	  2.9%
His (H)  10	  5.7%
Ile (I)   2	  1.1%
Leu (L)  16	  9.1%
Lys (K)  25	 14.3%
Met (M)   4	  2.3%
Phe (F)   8	  4.6%
Pro (P)   9	  5.1%
Ser (S)  19	 10.9%
Thr (T)   7	  4.0%
Trp (W)   2	  1.1%
Tyr (Y)   6	  3.4%
Val (V)   4	  2.3%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 12
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	       911
Hydrogen    H	      1443
Nitrogen    N	       277
Oxygen      O	       257
Sulfur      S	         4

Formula: C911H1443N277O257S4
Total number of atoms: 2892

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    19500   19600   19390   19060   18400
Abs 0.1% (=1 g/l)   0.950   0.955   0.945   0.929   0.897


Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 66.36
This classifies the protein as unstable.



Aliphatic index: 53.03

Grand average of hydropathicity (GRAVY): -1.221

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Sequence: Amino acid sequence.
CCP (C or -): coiled coil prediction from the ccp program (NCBI toolkit)
SEG (D or -): low complexity regions (possibly disordered) from SEG
PHDhtm (H or -): Transmembrane prediction from PHDhtm
TMHMM (H or -): Transmembrane prediction from TMHMM
PSIBLAST (3 or -): Regions potentially homologous to a protein of known 3D structure, according to PSIBLAST
Pred2ary (H, E, or -): Secondary structure prediction from Pred2ary

                 10        20        30        40        50        60        70        80
                  |         |         |         |         |         |         |         |
Sequence MSHKDFNGLQAPQLLSSSSPVAKKQSSHKLRHALKHARYLNHSSKRTLKHALELHEDNQVLLEKEGSPNFQDWLSKQPGV
CCP      --------------------------------------------------------------------------------
SEG      -------------DDDDDDDDDDDDDD-----------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary ---------------------------HHHHHHHHHHHH------HHHHHHH-------EEEE-----------------

                 90       100       110       120       130       140       150       160
                  |         |         |         |         |         |         |         |
Sequence NKTSLKYNKSLGSWISKESKPKKRFPPYFTYKGSKTTPEEAKALQQMKQSQKRFFHENMHSFLNEVAHNPMIQRFKQKQA
CCP      --------------------------------------------------------------------------------
SEG      --------------------------------------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary --EEEEE---------HHHHH-------E---------HHHHHHHHHHHHHHHHHHHHHHHHHHHHH--HHHHHHHHHHH

                170
                  |
Sequence KRAANTRQRTYKYRQ
CCP      ---------------
SEG      ---------------
PHDhtm   ---------------
TMHMM    ---------------
PSIBLAST ---------------
Pred2ary HHHHHHHHHHHH---

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