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Available sections:
| Target | log10(PSIBLAST E) | % identical | % coverage (of MP gene) | Latest Status |
|---|---|---|---|---|
| 1021B | -17.70 | 28.70 | 52.68 | Override suggest work stop (low sequencial homology to the known structures) |
| 1022B | -14.70 | 25.71 | 63.25 | In PDB (1G2I) |
ATGAAAAAACTCTTAGTCATTGTTTACCCTGACATGAATGATGTGGAATACACCAACACAATGGTGGTATTTGGTTTTGT TAAAGAATTACAAACCGTGATTTATCACCCTAACTTAAGTACAGTTAAGGGCAGTAATGGGGTAACTTTAGTAAACCAGA TTACTAGCAAGGTTAATCTGGAGGAATTTGATGGTGTCTTTATCCCTGGCGGTATGGGAGCTACCAAGGTATTAGACCAT GACCAACAGTTACTCGACACTATCCGTTACTTTAAGGATCATGACAAGTATGTCTTTGCCATTTGTGATACCCCCAACGT GTTGTAA
(109 codons) fields: [triplet] [frequency: per thousand] ([number]) UUU55.0( 6) UCU0.0( 0) UAU18.3( 2) UGU9.2( 1) UUC0.0( 0) UCC0.0( 0) UAC27.5( 3) UGC0.0( 0) UUA55.0( 6) UCA0.0( 0) UAA9.2( 1) UGA0.0( 0) UUG9.2( 1) UCG0.0( 0) UAG0.0( 0) UGG0.0( 0) CUU0.0( 0) CCU27.5( 3) CAU18.3( 2) CGU9.2( 1) CUC18.3( 2) CCC9.2( 1) CAC9.2( 1) CGC0.0( 0) CUA0.0( 0) CCA0.0( 0) CAA18.3( 2) CGA0.0( 0) CUG9.2( 1) CCG0.0( 0) CAG18.3( 2) CGG0.0( 0) AUU36.7( 4) ACU27.5( 3) AAU27.5( 3) AGU18.3( 2) AUC18.3( 2) ACC36.7( 4) AAC36.7( 4) AGC9.2( 1) AUA0.0( 0) ACA18.3( 2) AAA27.5( 3) AGA0.0( 0) AUG36.7( 4) ACG0.0( 0) AAG45.9( 5) AGG0.0( 0) GUU36.7( 4) GCU9.2( 1) GAU36.7( 4) GGU27.5( 3) GUC27.5( 3) GCC9.2( 1) GAC45.9( 5) GGC18.3( 2) GUA36.7( 4) GCA0.0( 0) GAA27.5( 3) GGA9.2( 1) GUG36.7( 4) GCG0.0( 0) GAG9.2( 1) GGG9.2( 1)
Note: This was generated using the standard codon usage table; UGA codons in MP/MP genes will show up as terminators ("*")
atgaaaaaactcttagtcattgtttaccctgacatgaatgatgtggaatacaccaacaca M K K L L V I V Y P D M N D V E Y T N T atggtggtatttggttttgttaaagaattacaaaccgtgatttatcaccctaacttaagt M V V F G F V K E L Q T V I Y H P N L S acagttaagggcagtaatggggtaactttagtaaaccagattactagcaaggttaatctg T V K G S N G V T L V N Q I T S K V N L gaggaatttgatggtgtctttatccctggcggtatgggagctaccaaggtattagaccat E E F D G V F I P G G M G A T K V L D H gaccaacagttactcgacactatccgttactttaaggatcatgacaagtatgtctttgcc D Q Q L L D T I R Y F K D H D K Y V F A atttgtgatacccccaacgtgttgtaa I C D T P N V L *
MKKLLVIVYPDMNDVEYTNTMVVFGFVKELQTVIYHPNLSTVKGSNGVTLVNQITSKVNLEEFDGVFIPGGMGATKVLDH DQQLLDTIRYFKDHDKYVFAICDTPNVL
This information was obtained using the Protein Parameters tool on the ExPASy Molecular Biology Server.
Number of amino acids: 108
Molecular weight: 12200.0
Theoretical pI: 5.14
Amino acid composition:
Ala (A) 2 1.9%
Arg (R) 1 0.9%
Asn (N) 7 6.5%
Asp (D) 9 8.3%
Cys (C) 1 0.9%
Gln (Q) 4 3.7%
Glu (E) 4 3.7%
Gly (G) 7 6.5%
His (H) 3 2.8%
Ile (I) 6 5.6%
Leu (L) 10 9.3%
Lys (K) 8 7.4%
Met (M) 4 3.7%
Phe (F) 6 5.6%
Pro (P) 4 3.7%
Ser (S) 3 2.8%
Thr (T) 9 8.3%
Trp (W) 0 0.0%
Tyr (Y) 5 4.6%
Val (V) 15 13.9%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 13
Total number of positively charged residues (Arg + Lys): 9
Atomic composition:
Carbon C 554
Hydrogen H 866
Nitrogen N 136
Oxygen O 163
Sulfur S 5
Formula: C554H866N136O163S5
Total number of atoms: 1724
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 7250 7000 6725 6400 6000
Abs 0.1% (=1 g/l) 0.594 0.574 0.551 0.525 0.492
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 7250 7000 6725 6400 6000
Abs 0.1% (=1 g/l) 0.594 0.574 0.551 0.525 0.492
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 5.40
This classifies the protein as stable.
Aliphatic index: 99.91
Grand average of hydropathicity (GRAVY): 0.044| Model | Percentage of MP Gene |
|---|---|
| Coiled Coil (CCP) | 0 |
| Disordered (SEG) | 0 |
| Transmembrane (PHDhtm) | 0 |
| Transmembrane (TMHMM) | 0 |
| Homologous to known structure (PSIBLAST) | 98 |
Sequence: Amino acid sequence.
CCP (C or -): coiled coil prediction from the ccp program (NCBI toolkit)
SEG (D or -): low complexity regions (possibly disordered) from SEG
PHDhtm (H or -): Transmembrane prediction from PHDhtm
TMHMM (H or -): Transmembrane prediction from TMHMM
PSIBLAST (3 or -): Regions potentially homologous to a protein of known 3D structure, according to PSIBLAST
Pred2ary (H, E, or -): Secondary structure prediction from Pred2ary
10 20 30 40 50 60 70 80
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Sequence MKKLLVIVYPDMNDVEYTNTMVVFGFVKELQTVIYHPNLSTVKGSNGVTLVNQITSKVNLEEFDGVFIPGGMGATKVLDH
CCP --------------------------------------------------------------------------------
SEG --------------------------------------------------------------------------------
PHDhtm --------------------------------------------------------------------------------
TMHMM --------------------------------------------------------------------------------
PSIBLAST -3333333333333333333333333333333333333333333333333333333333333333333333333333333
Pred2ary --EEEEEE-------HHHHHHHHHHH----EEEEE-----------------------------EEEE------------
90 100
| |
Sequence DQQLLDTIRYFKDHDKYVFAICDTPNVL
CCP ----------------------------
SEG ----------------------------
PHDhtm ----------------------------
TMHMM ----------------------------
PSIBLAST 3333333333333333333333333333
Pred2ary -HHHHHHHHHHHH----EEEEE------