• Potential Target Homologues
• DNA Sequence
• Codon Usage
• Translation
• Amino Acid Sequence
• Protein Parameters
• Predicted properties
• Prediction details

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ATGTTTAAATTAAAAATTAACAACTTCCAATTAGGTTTCAAACTCGTTCAGTGACCAGTGACGAACCACCTTCTTAAACA
TTTTTACGTTTTTCCAATCAATAATAAAGGAGGTCTTGCCATTAAACGCATAATTTCACTTGCTTTGTTTAAGAAAAGAC
TTAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTACGACATGGGATTTAATGGC
AATTTCAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAGCGAGTTCAAAGATGCCAACAATCA
GCGTCTCATAAAGATTGAAACTACTTTGGCTATCCAAGGCGAACAAATCAACAAATTGACTCAAACTGTTGAAAAGCAAG
GCGAACAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCAAGGAGCGAGCAAATTAGAGAACTTCAAGTGGAGCAAAAAG
CACAAAGACAAGAGTTTAATGCCCGCATGGATCGTTTGGAAAATCTTTTGGTGGAAAGTATAG

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(181 codons)
fields: [triplet] [frequency: per thousand] ([number])

UUU38.7(   7)  UCU0.0(   0)  UAU5.5(   1)  UGU5.5(   1)  
UUC27.6(   5)  UCC0.0(   0)  UAC11.0(   2)  UGC0.0(   0)  
UUA33.1(   6)  UCA5.5(   1)  UAA0.0(   0)  UGA5.5(   1)  
UUG16.6(   3)  UCG0.0(   0)  UAG5.5(   1)  UGG33.1(   6)  

CUU38.7(   7)  CCU5.5(   1)  CAU16.6(   3)  CGU5.5(   1)  
CUC11.0(   2)  CCC5.5(   1)  CAC11.0(   2)  CGC5.5(   1)  
CUA0.0(   0)  CCA11.0(   2)  CAA55.2(  10)  CGA5.5(   1)  
CUG0.0(   0)  CCG0.0(   0)  CAG16.6(   3)  CGG0.0(   0)  

AUU27.6(   5)  ACU22.1(   4)  AAU44.2(   8)  AGU11.0(   2)  
AUC33.1(   6)  ACC5.5(   1)  AAC44.2(   8)  AGC22.1(   4)  
AUA11.0(   2)  ACA0.0(   0)  AAA93.9(  17)  AGA5.5(   1)  
AUG22.1(   4)  ACG5.5(   1)  AAG44.2(   8)  AGG0.0(   0)  

GUU44.2(   8)  GCU11.0(   2)  GAU16.6(   3)  GGU22.1(   4)  
GUC0.0(   0)  GCC11.0(   2)  GAC11.0(   2)  GGC16.6(   3)  
GUA5.5(   1)  GCA5.5(   1)  GAA38.7(   7)  GGA16.6(   3)  
GUG11.0(   2)  GCG5.5(   1)  GAG16.6(   3)  GGG0.0(   0)  

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atgtttaaattaaaaattaacaacttccaattaggtttcaaactcgttcagtgaccagtg
 M  F  K  L  K  I  N  N  F  Q  L  G  F  K  L  V  Q  *  P  V 
acgaaccaccttcttaaacatttttacgtttttccaatcaataataaaggaggtcttgcc
 T  N  H  L  L  K  H  F  Y  V  F  P  I  N  N  K  G  G  L  A 
attaaacgcataatttcacttgctttgtttaagaaaagacttaacaaagataagattaat
 I  K  R  I  I  S  L  A  L  F  K  K  R  L  N  K  D  K  I  N 
aattgtcatgtttgggaagaagagttacctgatggtagctacgacatgggatttaatggc
 N  C  H  V  W  E  E  E  L  P  D  G  S  Y  D  M  G  F  N  G 
aatttcaaccatatggaaaaacgaaaaagtggttatgttacccaaaagcagtttagcgag
 N  F  N  H  M  E  K  R  K  S  G  Y  V  T  Q  K  Q  F  S  E 
ttcaaagatgccaacaatcagcgtctcataaagattgaaactactttggctatccaaggc
 F  K  D  A  N  N  Q  R  L  I  K  I  E  T  T  L  A  I  Q  G 
gaacaaatcaacaaattgactcaaactgttgaaaagcaaggcgaacaaatcaatcaatta
 E  Q  I  N  K  L  T  Q  T  V  E  K  Q  G  E  Q  I  N  Q  L 
gttcaagttgtgcttcttcaaggagcgagcaaattagagaacttcaagtggagcaaaaag
 V  Q  V  V  L  L  Q  G  A  S  K  L  E  N  F  K  W  S  K  K 
cacaaagacaagagtttaatgcccgcatggatcgtttggaaaatcttttggtggaaagta
 H  K  D  K  S  L  M  P  A  W  I  V  W  K  I  F  W  W  K  V 
tag
 * 

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MFKLKINNFQLGFKLVQWPVTNHLLKHFYVFPINNKGGLAIKRIISLALFKKRLNKDKINNCHVWEEELPDGSYDMGFNG
NFNHMEKRKSGYVTQKQFSEFKDANNQRLIKIETTLAIQGEQINKLTQTVEKQGEQINQLVQVVLLQGASKLENFKWSKK
HKDKSLMPAWIVWKIFWWKV

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This information was obtained using the Protein Parameters tool on the ExPASy Molecular Biology Server.

Number of amino acids: 180

Molecular weight: 21277.8

Theoretical pI: 9.88

Amino acid composition:

Ala (A)   6	  3.3%
Arg (R)   4	  2.2%
Asn (N)  16	  8.9%
Asp (D)   5	  2.8%
Cys (C)   1	  0.6%
Gln (Q)  13	  7.2%
Glu (E)  10	  5.6%
Gly (G)  10	  5.6%
His (H)   5	  2.8%
Ile (I)  13	  7.2%
Leu (L)  18	 10.0%
Lys (K)  25	 13.9%
Met (M)   4	  2.2%
Phe (F)  12	  6.7%
Pro (P)   4	  2.2%
Ser (S)   7	  3.9%
Thr (T)   6	  3.3%
Trp (W)   7	  3.9%
Tyr (Y)   3	  1.7%
Val (V)  11	  6.1%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	       982
Hydrogen    H	      1531
Nitrogen    N	       263
Oxygen      O	       256
Sulfur      S	         5

Formula: C982H1531N263O256S5
Total number of atoms: 3037

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    42150   43400   43655   43670   42800
Abs 0.1% (=1 g/l)   1.981   2.040   2.052   2.052   2.011



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    42150   43400   43655   43670   42800
Abs 0.1% (=1 g/l)   1.981   2.040   2.052   2.052   2.011


Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 23.47
This classifies the protein as stable.



Aliphatic index: 88.22

Grand average of hydropathicity (GRAVY): -0.491

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Sequence: Amino acid sequence.
CCP (C or -): coiled coil prediction from the ccp program (NCBI toolkit)
SEG (D or -): low complexity regions (possibly disordered) from SEG
PHDhtm (H or -): Transmembrane prediction from PHDhtm
TMHMM (H or -): Transmembrane prediction from TMHMM
PSIBLAST (3 or -): Regions potentially homologous to a protein of known 3D structure, according to PSIBLAST
Pred2ary (H, E, or -): Secondary structure prediction from Pred2ary

                 10        20        30        40        50        60        70        80
                  |         |         |         |         |         |         |         |
Sequence MFKLKINNFQLGFKLVQWPVTNHLLKHFYVFPINNKGGLAIKRIISLALFKKRLNKDKINNCHVWEEELPDGSYDMGFNG
CCP      --------------------------------------------------------------------------------
SEG      --------------------------------------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary --EEEE-------EEEE---HH------EEEEE------EEHHH-HHHHHHHHHHH-------EE--------EEEEE--

                 90       100       110       120       130       140       150       160
                  |         |         |         |         |         |         |         |
Sequence NFNHMEKRKSGYVTQKQFSEFKDANNQRLIKIETTLAIQGEQINKLTQTVEKQGEQINQLVQVVLLQGASKLENFKWSKK
CCP      ------------------------------------------CCCCCCCCCCCCCCCCCCCCCC----------------
SEG      ----------------------------------------------------------DDDDDDDDD-------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary -----------EEEHHHHHH-----HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH

                170       180
                  |         |
Sequence HKDKSLMPAWIVWKIFWWKV
CCP      --------------------
SEG      --------------------
PHDhtm   --------------------
TMHMM    --------------------
PSIBLAST --------------------
Pred2ary -------HHHHHHHHHH---

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