• Potential Target Homologues
• DNA Sequence
• Codon Usage
• Translation
• Amino Acid Sequence
• Protein Parameters
• Predicted properties
• Prediction details

Back to Top

ATGGACACTAAATTAACACCACCCTGGTTGCGTCAAGAAAATAACCTGCTCAACCCCGAAGTTACCCAACGTTTGTTTAT
CAACCAGGACAACATTCCGAACTTACCCACTGAAGCCCCCCAAGTTTATTTAGCCCGTTTTTTGGAATGAGTTGAACCAC
TAGTCTCGAAAGTAAAATTTGTGAAAGCACAAGCAGCAGTTCAGGATTACCTCAATTCTAAAGCCTGTGCACAAATTGAA
ACCATTATTGCAGAACGGGCGCAAAACACCCATTCGAGTTGGTTAGCTAACTGGTGGGTGCAGTACGCTTATTTAACTTC
GACTGGCCCGGTTAGTCCGGAAGTTAATGCACCCTATTATTTAGAGCTCCCGACTGTTGGTTGAAGTCAAGCCGAGTTAG
CAGCAGCATTGTCAGCCCAGCTATGACACATATACCAGCAAGTCCAAAAACGGCAGTTAACGAGCTTTAGTGTCAAGGAC
AAGCTGTTTTCACTCGATACCTTGCAATCAATCTTTGCTAGTTGCCAGATTCACCGCGCTGATGGGGATGTGTACTTTGT
TAATGATCAACCTGCTAACTTTATTGTGGTGATTAAGAACAATGTCTTCTATAAGTTAGTAATAGATAACAGTGGTAGCT
TAGAACAACTGCAAGCGCAACTGCAGTTAAGCTTTGTGCAAATCTTAGACAATGAGCTAAGTCACCCACCGCACTGGAAC
TTACTCACTGCTACCACAACCAAAGCGGAGTCACAAAGTTTACTCGATCAGTTGTGAGCGCAAAACCCAGAAATGTTGTT
GGACATCTATAACAGTGCCTTTATTGTGAACTTGGACAACGTCGAATTAACGACTCCCTTACAACTGTTGCGCAATTCGA
CATGAACCCCTAACTTTAACCGTTGGCACGCTAAGGGGATTCAGTTGGTCATTACTAAGAACGCTCAGTTAGTCATTCTA
GCGGATCACACTAGTTTTGACGGTTCTAGTGTAGCTACCCTAGCCAACATCTTTGTCTCTAAACTCCAAAAGGTCAACAC
TGAGGGTGCCAGCGCTTTAACACCGACCATGCTTTCCTTTCCAACAGTAGACCAGGATAAGCAAAAGTATTTTAAGCAAT
TGAGCAAAAATTTTAAGGACTATGTGTACAATGCCGTTATGTTTGAACTGAAATGGGACTGGTTTACGAAGCCACTTATC
AAAGCCAAAGGGATTAAGAACTCCGAAGCCTTTATCCATCTCTGTTACCAAATTGCCCAGTACCAAACGAACAAAAAGTT
GCAGAACACCTATGTGGCCGTGGACATGCGTCAGTACTTCCGGGGACGCACCGAGTGTCTTCGTCCTTTAAGTAAACAAT
CAGTTGCCTTTGTCAAACGCTACTGCAAAGATCCGAAAGGTACCTTAAAGCAGTTCCGAAAGTACTACCCAGCGATTGAA
AGTCTCCACTTTGAAAAAACCCGGTTAGCGCAAAAGGGTAGTGGGGTCAACCGTCACTTGCTGGGAGCATATTTGGCTTG
GAATGAACACCAAGACACAATAGCTAAACCCGCTTTATTTGAAACAAAGGCTTGAAAAACGATTGCTGCCAACCCACTGT
CCACATCGAGCATTGTGGACAAGTATTTGCGCAACTTTTCCTTCGACCCCGTAGAGCCAAACGGCATTGGTATTGCCTAT
GCCATTGATGACACTAATTTCCGTGCCATTCTCAGTGTGTATCAGCACAACTTACAGTACCTCAAAGATTGGATGAAACA
CTTTGAACAAACAGTGAAAACAATCCTTAAAACTCTTAAATAA

Back to Top

(601 codons)
fields: [triplet] [frequency: per thousand] ([number])

UUU39.9(  24)  UCU5.0(   3)  UAU21.6(  13)  UGU5.0(   3)  
UUC8.3(   5)  UCC6.7(   4)  UAC20.0(  12)  UGC3.3(   2)  
UUA38.3(  23)  UCA8.3(   5)  UAA1.7(   1)  UGA10.0(   6)  
UUG26.6(  16)  UCG8.3(   5)  UAG0.0(   0)  UGG16.6(  10)  

CUU8.3(   5)  CCU5.0(   3)  CAU3.3(   2)  CGU13.3(   8)  
CUC18.3(  11)  CCC13.3(   8)  CAC18.3(  11)  CGC8.3(   5)  
CUA8.3(   5)  CCA15.0(   9)  CAA44.9(  27)  CGA1.7(   1)  
CUG13.3(   8)  CCG11.6(   7)  CAG30.0(  18)  CGG6.7(   4)  

AUU33.3(  20)  ACU20.0(  12)  AAU18.3(  11)  AGU25.0(  15)  
AUC13.3(   8)  ACC21.6(  13)  AAC46.6(  28)  AGC10.0(   6)  
AUA5.0(   3)  ACA16.6(  10)  AAA41.6(  25)  AGA0.0(   0)  
AUG10.0(   6)  ACG8.3(   5)  AAG31.6(  19)  AGG0.0(   0)  

GUU16.6(  10)  GCU25.0(  15)  GAU20.0(  12)  GGU11.6(   7)  
GUC18.3(  11)  GCC30.0(  18)  GAC25.0(  15)  GGC3.3(   2)  
GUA8.3(   5)  GCA16.6(  10)  GAA30.0(  18)  GGA3.3(   2)  
GUG21.6(  13)  GCG11.6(   7)  GAG11.6(   7)  GGG6.7(   4)  

Back to Top

atggacactaaattaacaccaccctggttgcgtcaagaaaataacctgctcaaccccgaa
 M  D  T  K  L  T  P  P  W  L  R  Q  E  N  N  L  L  N  P  E 
gttacccaacgtttgtttatcaaccaggacaacattccgaacttacccactgaagccccc
 V  T  Q  R  L  F  I  N  Q  D  N  I  P  N  L  P  T  E  A  P 
caagtttatttagcccgttttttggaatgagttgaaccactagtctcgaaagtaaaattt
 Q  V  Y  L  A  R  F  L  E  *  V  E  P  L  V  S  K  V  K  F 
gtgaaagcacaagcagcagttcaggattacctcaattctaaagcctgtgcacaaattgaa
 V  K  A  Q  A  A  V  Q  D  Y  L  N  S  K  A  C  A  Q  I  E 
accattattgcagaacgggcgcaaaacacccattcgagttggttagctaactggtgggtg
 T  I  I  A  E  R  A  Q  N  T  H  S  S  W  L  A  N  W  W  V 
cagtacgcttatttaacttcgactggcccggttagtccggaagttaatgcaccctattat
 Q  Y  A  Y  L  T  S  T  G  P  V  S  P  E  V  N  A  P  Y  Y 
ttagagctcccgactgttggttgaagtcaagccgagttagcagcagcattgtcagcccag
 L  E  L  P  T  V  G  *  S  Q  A  E  L  A  A  A  L  S  A  Q 
ctatgacacatataccagcaagtccaaaaacggcagttaacgagctttagtgtcaaggac
 L  *  H  I  Y  Q  Q  V  Q  K  R  Q  L  T  S  F  S  V  K  D 
aagctgttttcactcgataccttgcaatcaatctttgctagttgccagattcaccgcgct
 K  L  F  S  L  D  T  L  Q  S  I  F  A  S  C  Q  I  H  R  A 
gatggggatgtgtactttgttaatgatcaacctgctaactttattgtggtgattaagaac
 D  G  D  V  Y  F  V  N  D  Q  P  A  N  F  I  V  V  I  K  N 
aatgtcttctataagttagtaatagataacagtggtagcttagaacaactgcaagcgcaa
 N  V  F  Y  K  L  V  I  D  N  S  G  S  L  E  Q  L  Q  A  Q 
ctgcagttaagctttgtgcaaatcttagacaatgagctaagtcacccaccgcactggaac
 L  Q  L  S  F  V  Q  I  L  D  N  E  L  S  H  P  P  H  W  N 
ttactcactgctaccacaaccaaagcggagtcacaaagtttactcgatcagttgtgagcg
 L  L  T  A  T  T  T  K  A  E  S  Q  S  L  L  D  Q  L  *  A 
caaaacccagaaatgttgttggacatctataacagtgcctttattgtgaacttggacaac
 Q  N  P  E  M  L  L  D  I  Y  N  S  A  F  I  V  N  L  D  N 
gtcgaattaacgactcccttacaactgttgcgcaattcgacatgaacccctaactttaac
 V  E  L  T  T  P  L  Q  L  L  R  N  S  T  *  T  P  N  F  N 
cgttggcacgctaaggggattcagttggtcattactaagaacgctcagttagtcattcta
 R  W  H  A  K  G  I  Q  L  V  I  T  K  N  A  Q  L  V  I  L 
gcggatcacactagttttgacggttctagtgtagctaccctagccaacatctttgtctct
 A  D  H  T  S  F  D  G  S  S  V  A  T  L  A  N  I  F  V  S 
aaactccaaaaggtcaacactgagggtgccagcgctttaacaccgaccatgctttccttt
 K  L  Q  K  V  N  T  E  G  A  S  A  L  T  P  T  M  L  S  F 
ccaacagtagaccaggataagcaaaagtattttaagcaattgagcaaaaattttaaggac
 P  T  V  D  Q  D  K  Q  K  Y  F  K  Q  L  S  K  N  F  K  D 
tatgtgtacaatgccgttatgtttgaactgaaatgggactggtttacgaagccacttatc
 Y  V  Y  N  A  V  M  F  E  L  K  W  D  W  F  T  K  P  L  I 
aaagccaaagggattaagaactccgaagcctttatccatctctgttaccaaattgcccag
 K  A  K  G  I  K  N  S  E  A  F  I  H  L  C  Y  Q  I  A  Q 
taccaaacgaacaaaaagttgcagaacacctatgtggccgtggacatgcgtcagtacttc
 Y  Q  T  N  K  K  L  Q  N  T  Y  V  A  V  D  M  R  Q  Y  F 
cggggacgcaccgagtgtcttcgtcctttaagtaaacaatcagttgcctttgtcaaacgc
 R  G  R  T  E  C  L  R  P  L  S  K  Q  S  V  A  F  V  K  R 
tactgcaaagatccgaaaggtaccttaaagcagttccgaaagtactacccagcgattgaa
 Y  C  K  D  P  K  G  T  L  K  Q  F  R  K  Y  Y  P  A  I  E 
agtctccactttgaaaaaacccggttagcgcaaaagggtagtggggtcaaccgtcacttg
 S  L  H  F  E  K  T  R  L  A  Q  K  G  S  G  V  N  R  H  L 
ctgggagcatatttggcttggaatgaacaccaagacacaatagctaaacccgctttattt
 L  G  A  Y  L  A  W  N  E  H  Q  D  T  I  A  K  P  A  L  F 
gaaacaaaggcttgaaaaacgattgctgccaacccactgtccacatcgagcattgtggac
 E  T  K  A  *  K  T  I  A  A  N  P  L  S  T  S  S  I  V  D 
aagtatttgcgcaacttttccttcgaccccgtagagccaaacggcattggtattgcctat
 K  Y  L  R  N  F  S  F  D  P  V  E  P  N  G  I  G  I  A  Y 
gccattgatgacactaatttccgtgccattctcagtgtgtatcagcacaacttacagtac
 A  I  D  D  T  N  F  R  A  I  L  S  V  Y  Q  H  N  L  Q  Y 
ctcaaagattggatgaaacactttgaacaaacagtgaaaacaatccttaaaactcttaaa
 L  K  D  W  M  K  H  F  E  Q  T  V  K  T  I  L  K  T  L  K 
taa
 * 

Back to Top

MDTKLTPPWLRQENNLLNPEVTQRLFINQDNIPNLPTEAPQVYLARFLEWVEPLVSKVKFVKAQAAVQDYLNSKACAQIE
TIIAERAQNTHSSWLANWWVQYAYLTSTGPVSPEVNAPYYLELPTVGWSQAELAAALSAQLWHIYQQVQKRQLTSFSVKD
KLFSLDTLQSIFASCQIHRADGDVYFVNDQPANFIVVIKNNVFYKLVIDNSGSLEQLQAQLQLSFVQILDNELSHPPHWN
LLTATTTKAESQSLLDQLWAQNPEMLLDIYNSAFIVNLDNVELTTPLQLLRNSTWTPNFNRWHAKGIQLVITKNAQLVIL
ADHTSFDGSSVATLANIFVSKLQKVNTEGASALTPTMLSFPTVDQDKQKYFKQLSKNFKDYVYNAVMFELKWDWFTKPLI
KAKGIKNSEAFIHLCYQIAQYQTNKKLQNTYVAVDMRQYFRGRTECLRPLSKQSVAFVKRYCKDPKGTLKQFRKYYPAIE
SLHFEKTRLAQKGSGVNRHLLGAYLAWNEHQDTIAKPALFETKAWKTIAANPLSTSSIVDKYLRNFSFDPVEPNGIGIAY
AIDDTNFRAILSVYQHNLQYLKDWMKHFEQTVKTILKTLK

Back to Top

This information was obtained using the Protein Parameters tool on the ExPASy Molecular Biology Server.

Number of amino acids: 600

Molecular weight: 68886.8

Theoretical pI: 9.01

Amino acid composition:

Ala (A)  50	  8.3%
Arg (R)  18	  3.0%
Asn (N)  39	  6.5%
Asp (D)  27	  4.5%
Cys (C)   5	  0.8%
Gln (Q)  45	  7.5%
Glu (E)  25	  4.2%
Gly (G)  15	  2.5%
His (H)  13	  2.2%
Ile (I)  31	  5.2%
Leu (L)  68	 11.3%
Lys (K)  44	  7.3%
Met (M)   6	  1.0%
Phe (F)  29	  4.8%
Pro (P)  27	  4.5%
Ser (S)  38	  6.3%
Thr (T)  40	  6.7%
Trp (W)  16	  2.7%
Tyr (Y)  25	  4.2%
Val (V)  39	  6.5%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      3149
Hydrogen    H	      4860
Nitrogen    N	       824
Oxygen      O	       892
Sulfur      S	        11

Formula: C3149H4860N824O892S11
Total number of atoms: 9736

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient   122940  124854  124425  123280  119840
Abs 0.1% (=1 g/l)   1.785   1.812   1.806   1.790   1.740



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient   122650  124600  124185  123040  119600
Abs 0.1% (=1 g/l)   1.780   1.809   1.803   1.786   1.736


Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.63
This classifies the protein as stable.



Aliphatic index: 91.53

Grand average of hydropathicity (GRAVY): -0.280

Back to Top

Back to Top

Sequence: Amino acid sequence.
CCP (C or -): coiled coil prediction from the ccp program (NCBI toolkit)
SEG (D or -): low complexity regions (possibly disordered) from SEG
PHDhtm (H or -): Transmembrane prediction from PHDhtm
TMHMM (H or -): Transmembrane prediction from TMHMM
PSIBLAST (3 or -): Regions potentially homologous to a protein of known 3D structure, according to PSIBLAST
Pred2ary (H, E, or -): Secondary structure prediction from Pred2ary

                 10        20        30        40        50        60        70        80
                  |         |         |         |         |         |         |         |
Sequence MDTKLTPPWLRQENNLLNPEVTQRLFINQDNIPNLPTEAPQVYLARFLEWVEPLVSKVKFVKAQAAVQDYLNSKACAQIE
CCP      --------------------------------------------------------------------------------
SEG      --------------------------------------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary ---------------------------------------HHHHHHHHHHHH-----HHHHHHHHHHHHHHH----HHHHH

                 90       100       110       120       130       140       150       160
                  |         |         |         |         |         |         |         |
Sequence TIIAERAQNTHSSWLANWWVQYAYLTSTGPVSPEVNAPYYLELPTVGWSQAELAAALSAQLWHIYQQVQKRQLTSFSVKD
CCP      --------------------------------------------------------------------------------
SEG      ------------------------------------------------DDDDDDDDDDDDD-------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary HHHHHHHH-----HHHHHHHHHHHHH-----EE--------------HHHHHHHHHHHHHHHHHHHHHH-----------

                170       180       190       200       210       220       230       240
                  |         |         |         |         |         |         |         |
Sequence KLFSLDTLQSIFASCQIHRADGDVYFVNDQPANFIVVIKNNVFYKLVIDNSGSLEQLQAQLQLSFVQILDNELSHPPHWN
CCP      --------------------------------------------------------------------------------
SEG      -----------------------------------------------------DDDDDDDDDD-----------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary ----HHHHHHHHH--------------------EEEEEE---EEEEEE---------HHHHHHHHHHHH-----------

                250       260       270       280       290       300       310       320
                  |         |         |         |         |         |         |         |
Sequence LLTATTTKAESQSLLDQLWAQNPEMLLDIYNSAFIVNLDNVELTTPLQLLRNSTWTPNFNRWHAKGIQLVITKNAQLVIL
CCP      --------------------------------------------------------------------------------
SEG      --------------------------------------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary ------HHHHHHHHHHH-----HHHHHHHHH--EEEEE------HHHHHHHHH-------------EEEEEE----EEEE

                330       340       350       360       370       380       390       400
                  |         |         |         |         |         |         |         |
Sequence ADHTSFDGSSVATLANIFVSKLQKVNTEGASALTPTMLSFPTVDQDKQKYFKQLSKNFKDYVYNAVMFELKWDWFTKPLI
CCP      --------------------------------------------------------------------------------
SEG      --------------------------------------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary --------HHHHHHHHHHHHH-----------------------HHHHHHHHHHHHHHHHHHH---EEEEEE--------

                410       420       430       440       450       460       470       480
                  |         |         |         |         |         |         |         |
Sequence KAKGIKNSEAFIHLCYQIAQYQTNKKLQNTYVAVDMRQYFRGRTECLRPLSKQSVAFVKRYCKDPKGTLKQFRKYYPAIE
CCP      --------------------------------------------------------------------------------
SEG      --------------------------------------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary --------HHHHHHHHHHHHHHH-------HHHHHHHHHH------------HHHHHHHHHH------HHHHHHHHHHHH

                490       500       510       520       530       540       550       560
                  |         |         |         |         |         |         |         |
Sequence SLHFEKTRLAQKGSGVNRHLLGAYLAWNEHQDTIAKPALFETKAWKTIAANPLSTSSIVDKYLRNFSFDPVEPNGIGIAY
CCP      --------------------------------------------------------------------------------
SEG      --------------------------------------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary HHHHHHHHHHH----HHHHHHHHHHHHHHH---------------------EEE-----------------------EEE

                570       580       590       600
                  |         |         |         |
Sequence AIDDTNFRAILSVYQHNLQYLKDWMKHFEQTVKTILKTLK
CCP      ----------------------------------------
SEG      ----------------------------------------
PHDhtm   ----------------------------------------
TMHMM    ----------------------------------------
PSIBLAST ----------------------------------------
Pred2ary EE----EEEEEE-----HHHHHHHHHHHHHHHHHHHHHH-

Back to Top