• Potential Target Homologues
• DNA Sequence
• Codon Usage
• Translation
• Amino Acid Sequence
• Protein Parameters
• Predicted properties
• Prediction details

Back to Top

ATGGTTAAGGTCTGAAAAATTGGGTTTGGGGTCTTTCTACCAACAGCGCTTTTATTCTCGGCCTGTTCGTTTAAGGACTA
TATTCCTACACCAAGCTTTAGGAAGGATTTCAGTACGGAGAACAATTTTGTTAAAAATAAGGTTCCCGGGAAGGATGACA
TTTACAGTAAATTTTACGATCTAACCTTTTCCTTAAACTTTGTCAACAACCAAGCCCAAGAGTTTGGTACCGGTTGGTTA
ATTGACTGGAAGGGTGATGAGAATAAGAATCTAAGCAAAAACAAAGAAGGACAAACGGCATCTCAAACCAGAAGTTCCTC
TGAACAAACTACCGATCAAGACGCTAACCTCTTTACTGCCTATATCGCCACTAACCTCCATGTCGCGGATGGTTTAAAGA
ATGATCAGGACTACGCTCCTTACAACAAGGATGGTTGGGGTCAACCATATCCTTACCAGCAAAAAACCCAATCCTTCTTA
TTAGGTAAGTACACCAAACCTAATGTCCAGTTAGTCAAGACTAACTATGAAAAGCCAGAGGATGCCGTGATTGAGCAGAA
GCTCAAGGAAGATTCGCTGTTGTTTATCCAAACGAGTACGTTACCTAAAACAGCATACGCAGCAATTGATCCGGTGAACT
TTAGCTATAACCCAACCCGTACCAACGGTTTTTGAACAGCCGGTAAATACAATGTTTACAATGGTGGTAACAGCATTGGT
AACTATGCTGATTTTGCTGTGATTGAAGTACCGTTAGTACTATCCAACCCCAATGATGCCAAGATCTACCAAGAGTGGAT
TAGACCAGCTACCCAAGCCTACAAATATTTAGGTGATGTAGAAGGTTTGTTTGCTAAGAAGGGCTATCGCAGTTACATCC
AAGATTTCTACCACTTATTGGGTTATCCGGTAACTAAGAACACTAAATCAGAGTTTATTTTGGGACAAAGTCAAGGTACT
GTGAACCACATGAGTTTTAGTAATGATGAGTCCAATACTACTAATACTATTACTAAAGCTAGCTTAACTCAGCAACCCCA
CAAGGAACAATCAGCTTACGTGGTACGTGAAAGTGGACTGCCAACCCTAACAATGAATGTGGACAAGTACACTGGTGCAA
AGGGAACTCACCTAGTTAACGTTGACCAAATTACTGACCTTAGTTTGGGTGATGGTTTAATTGACTTTGGTGGATTATCA
CGCTTTATCTTGCAGTACCACAATGTGAACTATAAGCAATTTGGTTATGGCACGATCTTGTGAGATACTAACTTTGGCGG
TGGTTCTTCTGGTAGTGCCATCTTTAACCAAAACAAGCAGATTAACAGTATTTACTTTGGAGCATTAGTTAATGTAACTA
CCGACAGAAACGAAAATGTGGGTTTAGGCTTAGGGCAAATTCTCCGTGCTCCCAATACCTTCAACTCTTCCCACGAAGTG
CCCTATTCTTATGACCTCATCTTTGGTGATGTTAACACCACCAATTTTTACGCCCAGTTTGCGAAAAAGCACAACACCCA
CATGTGGAGCAAAATTCAAAGTACGCAGAATGGAGAAATTGGTTTCCACAAAAATTCAAAAACCGGTCAACAGCGGCATT
AA

Back to Top

(534 codons)
fields: [triplet] [frequency: per thousand] ([number])

UUU48.7(  26)  UCU11.2(   6)  UAU24.3(  13)  UGU1.9(   1)  
UUC11.2(   6)  UCC11.2(   6)  UAC33.7(  18)  UGC0.0(   0)  
UUA31.8(  17)  UCA7.5(   4)  UAA1.9(   1)  UGA5.6(   3)  
UUG13.1(   7)  UCG5.6(   3)  UAG0.0(   0)  UGG9.4(   5)  

CUU3.7(   2)  CCU9.4(   5)  CAU3.7(   2)  CGU5.6(   3)  
CUC9.4(   5)  CCC9.4(   5)  CAC16.9(   9)  CGC3.7(   2)  
CUA11.2(   6)  CCA13.1(   7)  CAA43.1(  23)  CGA0.0(   0)  
CUG3.7(   2)  CCG5.6(   3)  CAG18.7(  10)  CGG1.9(   1)  

AUU33.7(  18)  ACU31.8(  17)  AAU37.5(  20)  AGU24.3(  13)  
AUC15.0(   8)  ACC30.0(  16)  AAC50.6(  27)  AGC11.2(   6)  
AUA0.0(   0)  ACA9.4(   5)  AAA30.0(  16)  AGA5.6(   3)  
AUG7.5(   4)  ACG11.2(   6)  AAG44.9(  24)  AGG1.9(   1)  

GUU15.0(   8)  GCU16.9(   9)  GAU35.6(  19)  GGU52.4(  28)  
GUC11.2(   6)  GCC18.7(  10)  GAC20.6(  11)  GGC7.5(   4)  
GUA11.2(   6)  GCA11.2(   6)  GAA20.6(  11)  GGA13.1(   7)  
GUG16.9(   9)  GCG5.6(   3)  GAG15.0(   8)  GGG7.5(   4)  

Back to Top

atggttaaggtctgaaaaattgggtttggggtctttctaccaacagcgcttttattctcg
 M  V  K  V  *  K  I  G  F  G  V  F  L  P  T  A  L  L  F  S 
gcctgttcgtttaaggactatattcctacaccaagctttaggaaggatttcagtacggag
 A  C  S  F  K  D  Y  I  P  T  P  S  F  R  K  D  F  S  T  E 
aacaattttgttaaaaataaggttcccgggaaggatgacatttacagtaaattttacgat
 N  N  F  V  K  N  K  V  P  G  K  D  D  I  Y  S  K  F  Y  D 
ctaaccttttccttaaactttgtcaacaaccaagcccaagagtttggtaccggttggtta
 L  T  F  S  L  N  F  V  N  N  Q  A  Q  E  F  G  T  G  W  L 
attgactggaagggtgatgagaataagaatctaagcaaaaacaaagaaggacaaacggca
 I  D  W  K  G  D  E  N  K  N  L  S  K  N  K  E  G  Q  T  A 
tctcaaaccagaagttcctctgaacaaactaccgatcaagacgctaacctctttactgcc
 S  Q  T  R  S  S  S  E  Q  T  T  D  Q  D  A  N  L  F  T  A 
tatatcgccactaacctccatgtcgcggatggtttaaagaatgatcaggactacgctcct
 Y  I  A  T  N  L  H  V  A  D  G  L  K  N  D  Q  D  Y  A  P 
tacaacaaggatggttggggtcaaccatatccttaccagcaaaaaacccaatccttctta
 Y  N  K  D  G  W  G  Q  P  Y  P  Y  Q  Q  K  T  Q  S  F  L 
ttaggtaagtacaccaaacctaatgtccagttagtcaagactaactatgaaaagccagag
 L  G  K  Y  T  K  P  N  V  Q  L  V  K  T  N  Y  E  K  P  E 
gatgccgtgattgagcagaagctcaaggaagattcgctgttgtttatccaaacgagtacg
 D  A  V  I  E  Q  K  L  K  E  D  S  L  L  F  I  Q  T  S  T 
ttacctaaaacagcatacgcagcaattgatccggtgaactttagctataacccaacccgt
 L  P  K  T  A  Y  A  A  I  D  P  V  N  F  S  Y  N  P  T  R 
accaacggtttttgaacagccggtaaatacaatgtttacaatggtggtaacagcattggt
 T  N  G  F  *  T  A  G  K  Y  N  V  Y  N  G  G  N  S  I  G 
aactatgctgattttgctgtgattgaagtaccgttagtactatccaaccccaatgatgcc
 N  Y  A  D  F  A  V  I  E  V  P  L  V  L  S  N  P  N  D  A 
aagatctaccaagagtggattagaccagctacccaagcctacaaatatttaggtgatgta
 K  I  Y  Q  E  W  I  R  P  A  T  Q  A  Y  K  Y  L  G  D  V 
gaaggtttgtttgctaagaagggctatcgcagttacatccaagatttctaccacttattg
 E  G  L  F  A  K  K  G  Y  R  S  Y  I  Q  D  F  Y  H  L  L 
ggttatccggtaactaagaacactaaatcagagtttattttgggacaaagtcaaggtact
 G  Y  P  V  T  K  N  T  K  S  E  F  I  L  G  Q  S  Q  G  T 
gtgaaccacatgagttttagtaatgatgagtccaatactactaatactattactaaagct
 V  N  H  M  S  F  S  N  D  E  S  N  T  T  N  T  I  T  K  A 
agcttaactcagcaaccccacaaggaacaatcagcttacgtggtacgtgaaagtggactg
 S  L  T  Q  Q  P  H  K  E  Q  S  A  Y  V  V  R  E  S  G  L 
ccaaccctaacaatgaatgtggacaagtacactggtgcaaagggaactcacctagttaac
 P  T  L  T  M  N  V  D  K  Y  T  G  A  K  G  T  H  L  V  N 
gttgaccaaattactgaccttagtttgggtgatggtttaattgactttggtggattatca
 V  D  Q  I  T  D  L  S  L  G  D  G  L  I  D  F  G  G  L  S 
cgctttatcttgcagtaccacaatgtgaactataagcaatttggttatggcacgatcttg
 R  F  I  L  Q  Y  H  N  V  N  Y  K  Q  F  G  Y  G  T  I  L 
tgagatactaactttggcggtggttcttctggtagtgccatctttaaccaaaacaagcag
 *  D  T  N  F  G  G  G  S  S  G  S  A  I  F  N  Q  N  K  Q 
attaacagtatttactttggagcattagttaatgtaactaccgacagaaacgaaaatgtg
 I  N  S  I  Y  F  G  A  L  V  N  V  T  T  D  R  N  E  N  V 
ggtttaggcttagggcaaattctccgtgctcccaataccttcaactcttcccacgaagtg
 G  L  G  L  G  Q  I  L  R  A  P  N  T  F  N  S  S  H  E  V 
ccctattcttatgacctcatctttggtgatgttaacaccaccaatttttacgcccagttt
 P  Y  S  Y  D  L  I  F  G  D  V  N  T  T  N  F  Y  A  Q  F 
gcgaaaaagcacaacacccacatgtggagcaaaattcaaagtacgcagaatggagaaatt
 A  K  K  H  N  T  H  M  W  S  K  I  Q  S  T  Q  N  G  E  I 
ggtttccacaaaaattcaaaaaccggtcaacagcggcattaa
 G  F  H  K  N  S  K  T  G  Q  Q  R  H  * 

Back to Top

MVKVWKIGFGVFLPTALLFSACSFKDYIPTPSFRKDFSTENNFVKNKVPGKDDIYSKFYDLTFSLNFVNNQAQEFGTGWL
IDWKGDENKNLSKNKEGQTASQTRSSSEQTTDQDANLFTAYIATNLHVADGLKNDQDYAPYNKDGWGQPYPYQQKTQSFL
LGKYTKPNVQLVKTNYEKPEDAVIEQKLKEDSLLFIQTSTLPKTAYAAIDPVNFSYNPTRTNGFWTAGKYNVYNGGNSIG
NYADFAVIEVPLVLSNPNDAKIYQEWIRPATQAYKYLGDVEGLFAKKGYRSYIQDFYHLLGYPVTKNTKSEFILGQSQGT
VNHMSFSNDESNTTNTITKASLTQQPHKEQSAYVVRESGLPTLTMNVDKYTGAKGTHLVNVDQITDLSLGDGLIDFGGLS
RFILQYHNVNYKQFGYGTILWDTNFGGGSSGSAIFNQNKQINSIYFGALVNVTTDRNENVGLGLGQILRAPNTFNSSHEV
PYSYDLIFGDVNTTNFYAQFAKKHNTHMWSKIQSTQNGEIGFHKNSKTGQQRH

Back to Top

This information was obtained using the Protein Parameters tool on the ExPASy Molecular Biology Server.

Number of amino acids: 533

Molecular weight: 59971.7

Theoretical pI: 7.69

Amino acid composition:

Ala (A)  28	  5.3%
Arg (R)  10	  1.9%
Asn (N)  47	  8.8%
Asp (D)  30	  5.6%
Cys (C)   1	  0.2%
Gln (Q)  33	  6.2%
Glu (E)  19	  3.6%
Gly (G)  43	  8.1%
His (H)  11	  2.1%
Ile (I)  26	  4.9%
Leu (L)  39	  7.3%
Lys (K)  40	  7.5%
Met (M)   4	  0.8%
Phe (F)  32	  6.0%
Pro (P)  20	  3.8%
Ser (S)  38	  7.1%
Thr (T)  44	  8.3%
Trp (W)   8	  1.5%
Tyr (Y)  31	  5.8%
Val (V)  29	  5.4%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      2707
Hydrogen    H	      4079
Nitrogen    N	       713
Oxygen      O	       825
Sulfur      S	         5

Formula: C2707H4079N713O825S5
Total number of atoms: 8329

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    88150   88200   86975   85200   82000
Abs 0.1% (=1 g/l)   1.470   1.471   1.450   1.421   1.367



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    88150   88200   86975   85200   82000
Abs 0.1% (=1 g/l)   1.470   1.471   1.450   1.421   1.367


Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.57
This classifies the protein as stable.



Aliphatic index: 68.59

Grand average of hydropathicity (GRAVY): -0.579

Back to Top

Back to Top

Sequence: Amino acid sequence.
CCP (C or -): coiled coil prediction from the ccp program (NCBI toolkit)
SEG (D or -): low complexity regions (possibly disordered) from SEG
PHDhtm (H or -): Transmembrane prediction from PHDhtm
TMHMM (H or -): Transmembrane prediction from TMHMM
PSIBLAST (3 or -): Regions potentially homologous to a protein of known 3D structure, according to PSIBLAST
Pred2ary (H, E, or -): Secondary structure prediction from Pred2ary

                 10        20        30        40        50        60        70        80
                  |         |         |         |         |         |         |         |
Sequence MVKVWKIGFGVFLPTALLFSACSFKDYIPTPSFRKDFSTENNFVKNKVPGKDDIYSKFYDLTFSLNFVNNQAQEFGTGWL
CCP      --------------------------------------------------------------------------------
SEG      --------------------------------------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary -EEEE-HHHHHHHHHHHHHH-------------E-------EEE-----------------EEEEEEE---------EEE

                 90       100       110       120       130       140       150       160
                  |         |         |         |         |         |         |         |
Sequence IDWKGDENKNLSKNKEGQTASQTRSSSEQTTDQDANLFTAYIATNLHVADGLKNDQDYAPYNKDGWGQPYPYQQKTQSFL
CCP      --------------------------------------------------------------------------------
SEG      --------------------------------------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary EE-----------------EEEEEE------------EEEEEE--HHHHH--------------------------EEEE

                170       180       190       200       210       220       230       240
                  |         |         |         |         |         |         |         |
Sequence LGKYTKPNVQLVKTNYEKPEDAVIEQKLKEDSLLFIQTSTLPKTAYAAIDPVNFSYNPTRTNGFWTAGKYNVYNGGNSIG
CCP      --------------------------------------------------------------------------------
SEG      --------------------------------------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary E------------------HHHHHH-------EEEE-------EEEE---------------------------------

                250       260       270       280       290       300       310       320
                  |         |         |         |         |         |         |         |
Sequence NYADFAVIEVPLVLSNPNDAKIYQEWIRPATQAYKYLGDVEGLFAKKGYRSYIQDFYHLLGYPVTKNTKSEFILGQSQGT
CCP      --------------------------------------------------------------------------------
SEG      --------------------------------------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary ----EEEEEE---------HHHHHHHHHHHHHHHHHH-------------------EEEE-----------EEE------

                330       340       350       360       370       380       390       400
                  |         |         |         |         |         |         |         |
Sequence VNHMSFSNDESNTTNTITKASLTQQPHKEQSAYVVRESGLPTLTMNVDKYTGAKGTHLVNVDQITDLSLGDGLIDFGGLS
CCP      --------------------------------------------------------------------------------
SEG      --------------------------------------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary --------------------------------------------------------EEE-------------EEEE----

                410       420       430       440       450       460       470       480
                  |         |         |         |         |         |         |         |
Sequence RFILQYHNVNYKQFGYGTILWDTNFGGGSSGSAIFNQNKQINSIYFGALVNVTTDRNENVGLGLGQILRAPNTFNSSHEV
CCP      --------------------------------------------------------------------------------
SEG      --------------------------------------------------------------------------------
PHDhtm   --------------------------------------------------------------------------------
TMHMM    --------------------------------------------------------------------------------
PSIBLAST --------------------------------------------------------------------------------
Pred2ary --EEE---HHHHH----EEEE-------------E-----EEEEEEEEEE-------------HHHHE------------

                490       500       510       520       530
                  |         |         |         |         |
Sequence PYSYDLIFGDVNTTNFYAQFAKKHNTHMWSKIQSTQNGEIGFHKNSKTGQQRH
CCP      -----------------------------------------------------
SEG      -----------------------------------------------------
PHDhtm   -----------------------------------------------------
TMHMM    -----------------------------------------------------
PSIBLAST -----------------------------------------------------
Pred2ary ---EEEEE-----HHHHHHHHHHHHHHH-----------EEEE----------

Back to Top