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This gene has the annotation "MG246 homolog, from M. genitalium".
The target was selected as part of JMC's first set.
Available sections:
| Experiment | Result | User | Experiment Date |
|---|---|---|---|
| Selected | Done | jmc | 2001-08-28 |
| Cloned | Done | blgold | 2002-08-28 |
| Expression tested | Done | dhshin | 2003-05-28 |
| Solubility tested | S | dhshin | 2003-05-28 |
| Purified | Done | dhshin | 2003-05-28 |
| Crystallized | Done | dhshin | 2003-05-28 |
| Diffraction quality crystals | Done | dhshin | 2003-06-18 |
| Native diffraction data | Done | dhshin | 2003-06-18 |
| Phasing diffraction data | Done | dhshin | 2004-05-07 |
| Traceable map | Done | dhshin | 2003-06-18 |
| Crystal structure | Done | dhshin | 2003-06-18 |
| In PDB | 1T71 | dhshin | 2004-05-07 |
| Suggested work stop | 18217 18217 | dallen | 2003-03-24 |
| MP gene | log10(PSIBLAST E) | % identical | % coverage (of MP gene) |
|---|---|---|---|
| MP487 / MPN349 | -116.00 | 100.00 | 100.00 |
ATGATGAATAGCATTAAATTCATTTTCCTAGGTGACGTCTATGGCAAGGCCGGACGTAATATTATTAAGAATAATCTAGC CCAACTCAAATCAAAGTATCAAGCTGATTTAGTCATTGTTAATGCCGAAAATACGACTCACGGTAAAGGACTTAGTTTAA AGCACTATGAGTTTCTAAAAGAGGCAGGCGTTAATTACATTACTATGGGTAATCATACCTGGTTTCAGAAGCTAGATTTA GCTGTTGTAATTAATAAAAAGGACTTAGTGCGTCCTTTAAATTTAGACACCAGTTTTGCTTTTCATAACTTAGGGCAAGG TAGTCTTGTATTTGAGTTTAATAAAGCAAAGATTAGGATAACTAATTTACTGGGTACTAGTGTGCCCTTACCCTTTAAAA CAACTAATCCCTTTAAAGTTTTAAAAGAACTAATTTTAAAGCGCGATTGTGATCTTCATATAGTTGATTTTCACGCTGAA ACCACCAGTGAAAAAAACGCCTTTTGCATGGCTTTTGATGGTTATGTCACAACCATTTTCGGCACCCACACCCACGTGCC TAGTGCTGATTTAAGAATCACCCCCAAAGGTAGTGCTTACATTACCGATGTCGGGATGTGTGGTCCGGGATTTGGTAGTG TTATTGGTGCTAACCCCGAACAGTCGATCAGACTCTTTTGTGCTGGTAGCAGAGAACACTTTGAGGTATCTAAATGCGGA GCACAACTCAATGGTGTCTTCTTTGAGGTTGATGTAAATACAAAAAAGGTGATTAAAACAGAGGCGATTAGGATTGTGGA GGACGATCCTAGATACTTAAAACAAGACTACTTTAACCTAATTTAA
(282 codons) fields: [triplet] [frequency: per thousand] ([number]) UUU56.7( 16) UCU3.5( 1) UAU14.2( 4) UGU10.6( 3) UUC14.2( 4) UCC0.0( 0) UAC14.2( 4) UGC7.1( 2) UUA46.1( 13) UCA3.5( 1) UAA3.5( 1) UGA0.0( 0) UUG0.0( 0) UCG3.5( 1) UAG0.0( 0) UGG3.5( 1) CUU10.6( 3) CCU10.6( 3) CAU10.6( 3) CGU7.1( 2) CUC10.6( 3) CCC17.7( 5) CAC21.3( 6) CGC3.5( 1) CUA21.3( 6) CCA0.0( 0) CAA17.7( 5) CGA0.0( 0) CUG3.5( 1) CCG3.5( 1) CAG7.1( 2) CGG0.0( 0) AUU56.7( 16) ACU17.7( 5) AAU53.2( 15) AGU28.4( 8) AUC7.1( 2) ACC31.9( 9) AAC14.2( 4) AGC7.1( 2) AUA7.1( 2) ACA14.2( 4) AAA53.2( 15) AGA14.2( 4) AUG17.7( 5) ACG3.5( 1) AAG31.9( 9) AGG7.1( 2) GUU24.8( 7) GCU31.9( 9) GAU35.5( 10) GGU42.6( 12) GUC17.7( 5) GCC14.2( 4) GAC17.7( 5) GGC10.6( 3) GUA14.2( 4) GCA10.6( 3) GAA21.3( 6) GGA14.2( 4) GUG17.7( 5) GCG3.5( 1) GAG24.8( 7) GGG7.1( 2)
Note: These were generated using Hisao's algorithm, and do not take into account possible mispriming, self-complementarity, or requirements for mutations anywhere in the sequences. The -R1 primers are for the N-terminal end, and begin with an NdeI restriction site (CATATG). The -R2 primers are for the C-terminal end, and begin with a BamHI site (GGATCC) if the target has no other BamHI sites. If the target does have a BamHI site, the -R2 primer wil begin with a BglII site (AGATCT), unless the target has no BglII sites. If the target has both sites, the design program gives up and doesn't prepend a restriction site to the -R2 primer!
Predicted Tm was obtained using the Tm determination tool at the Virtual Genome Center.
| Primer ID | Predicted Tm | sequence |
|---|---|---|
| 1150B-R1 | 60.80 | CATATGATGAATAGCATTAAATTCATTTTCC |
| 1150B-R2 | 61.70 | GGATCCTTAAATTAGGTTAAAGTAGTCTTGTTTTAAGTATCTAGG |
| 1150B-R10 | 64.30 | GGCGGTGGTGGCGGCATGATGAATAGCATTAAATTCATTTTCCTAGG |
| 1150B-R11 | 63.00 | GTTCTTCTCCTTTGCGCCCCTAAATTAGGTTAAAGTAGTCTTGTTTTAAGTATCTAGGATC |
Note: This was generated using the standard codon usage table; UGA codons in MP/MP genes will show up as terminators ("*")
atgatgaatagcattaaattcattttcctaggtgacgtctatggcaaggccggacgtaat M M N S I K F I F L G D V Y G K A G R N attattaagaataatctagcccaactcaaatcaaagtatcaagctgatttagtcattgtt I I K N N L A Q L K S K Y Q A D L V I V aatgccgaaaatacgactcacggtaaaggacttagtttaaagcactatgagtttctaaaa N A E N T T H G K G L S L K H Y E F L K gaggcaggcgttaattacattactatgggtaatcatacctggtttcagaagctagattta E A G V N Y I T M G N H T W F Q K L D L gctgttgtaattaataaaaaggacttagtgcgtcctttaaatttagacaccagttttgct A V V I N K K D L V R P L N L D T S F A tttcataacttagggcaaggtagtcttgtatttgagtttaataaagcaaagattaggata F H N L G Q G S L V F E F N K A K I R I actaatttactgggtactagtgtgcccttaccctttaaaacaactaatccctttaaagtt T N L L G T S V P L P F K T T N P F K V ttaaaagaactaattttaaagcgcgattgtgatcttcatatagttgattttcacgctgaa L K E L I L K R D C D L H I V D F H A E accaccagtgaaaaaaacgccttttgcatggcttttgatggttatgtcacaaccattttc T T S E K N A F C M A F D G Y V T T I F ggcacccacacccacgtgcctagtgctgatttaagaatcacccccaaaggtagtgcttac G T H T H V P S A D L R I T P K G S A Y attaccgatgtcgggatgtgtggtccgggatttggtagtgttattggtgctaaccccgaa I T D V G M C G P G F G S V I G A N P E cagtcgatcagactcttttgtgctggtagcagagaacactttgaggtatctaaatgcgga Q S I R L F C A G S R E H F E V S K C G gcacaactcaatggtgtcttctttgaggttgatgtaaatacaaaaaaggtgattaaaaca A Q L N G V F F E V D V N T K K V I K T gaggcgattaggattgtggaggacgatcctagatacttaaaacaagactactttaaccta E A I R I V E D D P R Y L K Q D Y F N L atttaa I *
MMNSIKFIFLGDVYGKAGRNIIKNNLAQLKSKYQADLVIVNAENTTHGKGLSLKHYEFLKEAGVNYITMGNHTWFQKLDL AVVINKKDLVRPLNLDTSFAFHNLGQGSLVFEFNKAKIRITNLLGTSVPLPFKTTNPFKVLKELILKRDCDLHIVDFHAE TTSEKNAFCMAFDGYVTTIFGTHTHVPSADLRITPKGSAYITDVGMCGPGFGSVIGANPEQSIRLFCAGSREHFEVSKCG AQLNGVFFEVDVNTKKVIKTEAIRIVEDDPRYLKQDYFNLI
This information was obtained using the Protein Parameters tool on the ExPASy Molecular Biology Server.
Number of amino acids: 281
Molecular weight: 31431.2
Theoretical pI: 8.83
Amino acid composition:
Ala (A) 17 6.0%
Arg (R) 9 3.2%
Asn (N) 19 6.8%
Asp (D) 15 5.3%
Cys (C) 5 1.8%
Gln (Q) 7 2.5%
Glu (E) 13 4.6%
Gly (G) 21 7.5%
His (H) 9 3.2%
Ile (I) 20 7.1%
Leu (L) 26 9.3%
Lys (K) 24 8.5%
Met (M) 5 1.8%
Phe (F) 20 7.1%
Pro (P) 9 3.2%
Ser (S) 13 4.6%
Thr (T) 19 6.8%
Trp (W) 1 0.4%
Tyr (Y) 8 2.8%
Val (V) 21 7.5%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 33
Atomic composition:
Carbon C 1425
Hydrogen H 2233
Nitrogen N 377
Oxygen O 404
Sulfur S 10
Formula: C1425H2233N377O404S10
Total number of atoms: 4449
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 17290 17054 16660 16170 15440
Abs 0.1% (=1 g/l) 0.550 0.543 0.530 0.514 0.491
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 17000 16800 16420 15930 15200
Abs 0.1% (=1 g/l) 0.541 0.535 0.522 0.507 0.484
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 16.84
This classifies the protein as stable.
Aliphatic index: 91.57
Grand average of hydropathicity (GRAVY): -0.086