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This gene has the annotation "hypothetical protein".
The target was selected as part of JMC's first set.
Available sections:
| Experiment | Result | User | Experiment Date |
|---|---|---|---|
| Selected | Done | jmc | 2001-08-28 |
| Cloned | Done | r_kim | 2002-01-15 |
| Expression tested | Done | r_kim | 2002-01-15 |
| Solubility tested | S | r_kim | 2002-01-15 |
| Purified | Done | r_kim | 2002-02-26 |
| Crystallized | Done | dhshin | 2002-08-27 |
| Diffraction quality crystals | Done | dhshin | 2002-08-27 |
| Native diffraction data | Done | dhshin | 2002-08-27 |
| Phasing diffraction data | Done | dhshin | 2002-08-27 |
| Traceable map | Done | dhshin | 2002-08-27 |
| Crystal structure | Done | dhshin | 2002-08-27 |
| NMR characterization | Done | dewemmer | 2002-10-11 |
| HSQC | Done | dewemmer | 2002-10-11 |
| In PDB | 1S12 | dhshin | 2004-01-05 |
| Suggested work stop | 19183 19183 | dallen | 2003-05-20 |
| Override suggest work stop | Structure solved | dhshin | 2003-06-05 |
ATGATAAAAGTAACTGTGACTAATTCTTTTTTTGAAGTAACGGGTCATGCACCAGACAAAACACTGTGCGCTTCAGTTAG TCTTTTGACGCAGCATGTGGCGAACTTTCTGAAAGCCGAGAAAAAAGCGAAAATAAAGAAGGAATCCGGTTATCTGAAAG TGAAATTTGAAGAACTCGAAAACTGCGAAGTGAAGGTGCTAGCGGCTATGGTGAGGTCTTTGAAAGAACTCGAACAGAAG TTTCCTTCTCAGATCAGAGTGGAGGTGATCGACAATGGCTCATAA
(95 codons) fields: [triplet] [frequency: per thousand] ([number]) UUU52.6( 5) UCU31.6( 3) UAU10.5( 1) UGU0.0( 0) UUC0.0( 0) UCC10.5( 1) UAC0.0( 0) UGC21.1( 2) UUA0.0( 0) UCA21.1( 2) UAA10.5( 1) UGA0.0( 0) UUG21.1( 2) UCG0.0( 0) UAG0.0( 0) UGG0.0( 0) CUU10.5( 1) CCU10.5( 1) CAU21.1( 2) CGU0.0( 0) CUC21.1( 2) CCC0.0( 0) CAC0.0( 0) CGC0.0( 0) CUA10.5( 1) CCA10.5( 1) CAA0.0( 0) CGA0.0( 0) CUG31.6( 3) CCG0.0( 0) CAG31.6( 3) CGG0.0( 0) AUU0.0( 0) ACU21.1( 2) AAU21.1( 2) AGU10.5( 1) AUC21.1( 2) ACC0.0( 0) AAC21.1( 2) AGC0.0( 0) AUA21.1( 2) ACA10.5( 1) AAA94.7( 9) AGA10.5( 1) AUG21.1( 2) ACG21.1( 2) AAG42.1( 4) AGG10.5( 1) GUU10.5( 1) GCU21.1( 2) GAU0.0( 0) GGU21.1( 2) GUC0.0( 0) GCC10.5( 1) GAC21.1( 2) GGC10.5( 1) GUA21.1( 2) GCA10.5( 1) GAA84.2( 8) GGA0.0( 0) GUG84.2( 8) GCG31.6( 3) GAG21.1( 2) GGG0.0( 0)
Note: These were generated using Hisao's algorithm, and do not take into account possible mispriming, self-complementarity, or requirements for mutations anywhere in the sequences. The -R1 primers are for the N-terminal end, and begin with an NdeI restriction site (CATATG). The -R2 primers are for the C-terminal end, and begin with a BamHI site (GGATCC) if the target has no other BamHI sites. If the target does have a BamHI site, the -R2 primer wil begin with a BglII site (AGATCT), unless the target has no BglII sites. If the target has both sites, the design program gives up and doesn't prepend a restriction site to the -R2 primer!
Predicted Tm was obtained using the Tm determination tool at the Virtual Genome Center.
| Primer ID | Predicted Tm | sequence |
|---|---|---|
| 1167B-R1 | 61.70 | CATATGATAAAAGTAACTGTGACTAATTCTTTTTTTG |
| 1167B-R2 | 62.00 | GGATCCTTATGAGCCATTGTCGATCACC |
| 1167B-R10 | 63.50 | GGCGGTGGTGGCGGCATGATAAAAGTAACTGTGACTAATTCTTTTTTTGAA |
| 1167B-R11 | 63.40 | GTTCTTCTCCTTTGCGCCCCTATGAGCCATTGTCGATCACCTC |
Note: This was generated using the standard codon usage table; UGA codons in MP/MP genes will show up as terminators ("*")
atgataaaagtaactgtgactaattctttttttgaagtaacgggtcatgcaccagacaaa M I K V T V T N S F F E V T G H A P D K acactgtgcgcttcagttagtcttttgacgcagcatgtggcgaactttctgaaagccgag T L C A S V S L L T Q H V A N F L K A E aaaaaagcgaaaataaagaaggaatccggttatctgaaagtgaaatttgaagaactcgaa K K A K I K K E S G Y L K V K F E E L E aactgcgaagtgaaggtgctagcggctatggtgaggtctttgaaagaactcgaacagaag N C E V K V L A A M V R S L K E L E Q K tttccttctcagatcagagtggaggtgatcgacaatggctcataa F P S Q I R V E V I D N G S *
MIKVTVTNSFFEVTGHAPDKTLCASVSLLTQHVANFLKAEKKAKIKKESGYLKVKFEELENCEVKVLAAMVRSLKELEQK FPSQIRVEVIDNGS
This information was obtained using the Protein Parameters tool on the ExPASy Molecular Biology Server.
Number of amino acids: 94
Molecular weight: 10540.3
Theoretical pI: 8.93
Amino acid composition:
Ala (A) 7 7.4%
Arg (R) 2 2.1%
Asn (N) 4 4.3%
Asp (D) 2 2.1%
Cys (C) 2 2.1%
Gln (Q) 3 3.2%
Glu (E) 10 10.6%
Gly (G) 3 3.2%
His (H) 2 2.1%
Ile (I) 4 4.3%
Leu (L) 9 9.6%
Lys (K) 13 13.8%
Met (M) 2 2.1%
Phe (F) 5 5.3%
Pro (P) 2 2.1%
Ser (S) 7 7.4%
Thr (T) 5 5.3%
Trp (W) 0 0.0%
Tyr (Y) 1 1.1%
Val (V) 11 11.7%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 12
Total number of positively charged residues (Arg + Lys): 15
Atomic composition:
Carbon C 472
Hydrogen H 776
Nitrogen N 124
Oxygen O 139
Sulfur S 4
Formula: C472H776N124O139S4
Total number of atoms: 1515
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 1595 1527 1465 1400 1320
Abs 0.1% (=1 g/l) 0.151 0.145 0.139 0.133 0.125
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 1450 1400 1345 1280 1200
Abs 0.1% (=1 g/l) 0.138 0.133 0.128 0.121 0.114
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 9.46
This classifies the protein as stable.
Aliphatic index: 95.32
Grand average of hydropathicity (GRAVY): -0.145