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This gene has the annotation "conserved hypothetical protein".
The target was selected as part of pre-BSGC set.
Available sections:
| Experiment | Result | User | Experiment Date |
|---|---|---|---|
| Selected | Done | jmc | 2001-09-10 |
| Cloned | Done | abwaight | 2001-09-21 |
| Expression tested | Done | abwaight | 2001-09-21 |
| Solubility tested | S | abwaight | 2001-09-21 |
| Purified | Done | abwaight | 2001-09-21 |
| Crystallized | Done | abwaight | 2001-09-21 |
| Diffraction quality crystals | Done | abwaight | 2001-09-21 |
| Phasing diffraction data | Done | abwaight | 2001-09-21 |
| Traceable map | Done | abwaight | 2001-09-21 |
| Crystal structure | Done | abwaight | 2001-09-21 |
| In PDB | 1JEO | abwaight | 2001-09-21 |
| Biochemical function | Done | dhshin | 2001-09-20 |
GTGTCGAAATTGGAAGAACTTGATATAGTATCTAACAATATATTGATATTAAAAAAATTCTATACAAACGATGAGTGGAA AAATAAACTGGATTCTTTAATTGATAGGATTATAAAAGCTAAAAAAATTTTTATTTTTGGAGTAGGTAGGAGTGGATATA TTGGAAGATGTTTTGCCATGAGATTAATGCATCTTGGTTTTAAATCATATTTTGTTGGGGAAACTACAACTCCTTCCTAT GAAAAAGATGATTTACTAATTTTAATATCGGGTAGTGGAAGAACAGAGAGTGTTTTAACAGTAGCTAAAAAGGCAAAGAA TATAAATAACAACATTATCGCAATTGTATGTGAATGTGGAAATGTAGTAGAGTTTGCTGATTTAACAATTCCTTTAGAAG TGAAGAAATCAAAATATTTACCAATGGGAACCACTTTTGAAGAAACAGCTTTGATATTTTTAGATTTAGTCATAGCTGAG ATTATGAAAAGATTGAATTTAGATGAAAGTGAAATAATAAAGAGACATTGTAATTTACTTTAA
(181 codons) fields: [triplet] [frequency: per thousand] ([number]) UUU44.2( 8) UCU11.0( 2) UAU27.6( 5) UGU22.1( 4) UUC5.5( 1) UCC5.5( 1) UAC0.0( 0) UGC0.0( 0) UUA71.8( 13) UCA11.0( 2) UAA5.5( 1) UGA0.0( 0) UUG22.1( 4) UCG11.0( 2) UAG0.0( 0) UGG5.5( 1) CUU16.6( 3) CCU11.0( 2) CAU11.0( 2) CGU0.0( 0) CUC0.0( 0) CCC0.0( 0) CAC0.0( 0) CGC0.0( 0) CUA5.5( 1) CCA5.5( 1) CAA0.0( 0) CGA0.0( 0) CUG5.5( 1) CCG0.0( 0) CAG0.0( 0) CGG0.0( 0) AUU55.2( 10) ACU16.6( 3) AAU38.7( 7) AGU22.1( 4) AUC5.5( 1) ACC5.5( 1) AAC22.1( 4) AGC0.0( 0) AUA55.2( 10) ACA33.1( 6) AAA77.3( 14) AGA27.6( 5) AUG22.1( 4) ACG0.0( 0) AAG22.1( 4) AGG11.0( 2) GUU11.0( 2) GCU27.6( 5) GAU49.7( 9) GGU16.6( 3) GUC5.5( 1) GCC5.5( 1) GAC0.0( 0) GGC0.0( 0) GUA33.1( 6) GCA11.0( 2) GAA55.2( 10) GGA33.1( 6) GUG11.0( 2) GCG0.0( 0) GAG22.1( 4) GGG5.5( 1)
Note: These were generated using Hisao's algorithm, and do not take into account possible mispriming, self-complementarity, or requirements for mutations anywhere in the sequences. The -R1 primers are for the N-terminal end, and begin with an NdeI restriction site (CATATG). The -R2 primers are for the C-terminal end, and begin with a BamHI site (GGATCC) if the target has no other BamHI sites. If the target does have a BamHI site, the -R2 primer wil begin with a BglII site (AGATCT), unless the target has no BglII sites. If the target has both sites, the design program gives up and doesn't prepend a restriction site to the -R2 primer!
Predicted Tm was obtained using the Tm determination tool at the Virtual Genome Center.
| Primer ID | Predicted Tm | sequence |
|---|---|---|
| 1199B-R1 | 60.10 | CATATGTCGAAATTGGAAGAACTTGATAT |
| 1199B-R2 | 60.10 | GGATCCTTAAAGTAAATTACAATGTCTCTTTATTATTTCACT |
| 1199B-R10 | 63.50 | GGCGGTGGTGGCGGCATGTCGAAATTGGAAGAACTTGATATAGTATCT |
| 1199B-R11 | 63.50 | GTTCTTCTCCTTTGCGCCCCTAAAGTAAATTACAATGTCTCTTTATTATTTCACTTTCA |
Note: This was generated using the standard codon usage table; UGA codons in MP/MP genes will show up as terminators ("*")
gtgtcgaaattggaagaacttgatatagtatctaacaatatattgatattaaaaaaattc V S K L E E L D I V S N N I L I L K K F tatacaaacgatgagtggaaaaataaactggattctttaattgataggattataaaagct Y T N D E W K N K L D S L I D R I I K A aaaaaaatttttatttttggagtaggtaggagtggatatattggaagatgttttgccatg K K I F I F G V G R S G Y I G R C F A M agattaatgcatcttggttttaaatcatattttgttggggaaactacaactccttcctat R L M H L G F K S Y F V G E T T T P S Y gaaaaagatgatttactaattttaatatcgggtagtggaagaacagagagtgttttaaca E K D D L L I L I S G S G R T E S V L T gtagctaaaaaggcaaagaatataaataacaacattatcgcaattgtatgtgaatgtgga V A K K A K N I N N N I I A I V C E C G aatgtagtagagtttgctgatttaacaattcctttagaagtgaagaaatcaaaatattta N V V E F A D L T I P L E V K K S K Y L ccaatgggaaccacttttgaagaaacagctttgatatttttagatttagtcatagctgag P M G T T F E E T A L I F L D L V I A E attatgaaaagattgaatttagatgaaagtgaaataataaagagacattgtaatttactt I M K R L N L D E S E I I K R H C N L L taa *
MSKLEELDIVSNNILILKKFYTNDEWKNKLDSLIDRIIKAKKIFIFGVGRSGYIGRCFAMRLMHLGFKSYFVGETTTPSY EKDDLLILISGSGRTESVLTVAKKAKNINNNIIAIVCECGNVVEFADLTIPLEVKKSKYLPMGTTFEETALIFLDLVIAE IMKRLNLDESEIIKRHCNLL
This information was obtained using the Protein Parameters tool on the ExPASy Molecular Biology Server.
Number of amino acids: 180
Molecular weight: 20443.0
Theoretical pI: 8.28
Amino acid composition:
Ala (A) 8 4.4%
Arg (R) 7 3.9%
Asn (N) 11 6.1%
Asp (D) 9 5.0%
Cys (C) 4 2.2%
Gln (Q) 0 0.0%
Glu (E) 14 7.8%
Gly (G) 10 5.6%
His (H) 2 1.1%
Ile (I) 21 11.7%
Leu (L) 22 12.2%
Lys (K) 18 10.0%
Met (M) 5 2.8%
Phe (F) 9 5.0%
Pro (P) 3 1.7%
Ser (S) 11 6.1%
Thr (T) 10 5.6%
Trp (W) 1 0.6%
Tyr (Y) 5 2.8%
Val (V) 10 5.6%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 926
Hydrogen H 1505
Nitrogen N 235
Oxygen O 264
Sulfur S 9
Formula: C926H1505N235O264S9
Total number of atoms: 2939
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 12940 12854 12625 12330 11840
Abs 0.1% (=1 g/l) 0.633 0.629 0.618 0.603 0.579
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 12650 12600 12385 12090 11600
Abs 0.1% (=1 g/l) 0.619 0.616 0.606 0.591 0.567
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.75
This classifies the protein as unstable.
Aliphatic index: 113.72
Grand average of hydropathicity (GRAVY): 0.112