Target info for 1200B

This gene has the annotation "180aa long hypothetical pyrazinamidase/nicotinamidase".

The target was selected as part of pre-BSGC set.

Available sections:

Experimental status of 1200B

Experiment	Result	User	Experiment Date
Selected	Done	jmc	2001-09-10
Cloned	Done	abwaight	2001-09-21
Expression tested	Done	abwaight	2001-09-21
Solubility tested	S	abwaight	2001-09-21
Purified	Done	abwaight	2001-09-21
Crystallized	Done	abwaight	2001-09-21
Diffraction quality crystals	Done	abwaight	2001-09-21
Phasing diffraction data	Done	abwaight	2001-09-21
Traceable map	Done	abwaight	2001-09-21
Crystal structure	Done	abwaight	2001-09-21
In PDB	1ILW	abwaight	2001-09-21
Biochemical function	Done	dhshin	2001-09-20

Potential MP Homologues of 1200B

1200B DNA sequence

ATGCCTGAAGAAGCCTTAATAGTTGTTGATATGCAGAGAGATTTCATGCCTGGAGGAGCTCTTCCGGTTCCTGAGGGAGA
TAAGATAATTCCAAAGGTTAACGAGTACATAAGGAAGTTTAAGGAAAAGGGAGCTTTAATAGTGGCCACAAGGGATTGGC
ATCCTGAAAATCACATAAGCTTTAGGGAGAGGGGAGGCCCCTGGCCCAGGCATTGCGTTCAAAACACTCCTGGTGCAGAG
TTCGTGGTAGATTTACCGGAAGATGCCGTGATCATTTCGAAAGCTACAGAGCCAGATAAAGAAGCATATTCTGGCTTTGA
AGGTACAGATCTTGCGAAAATTCTAAGAGGGAATGGAGTTAAGAGAGTTTATATCTGTGGTGTTGCTACGGAGTACTGCG
TCAGGGCAACGGCCTTAGACGCTCTTAAGCATGGGTTTGAAGTCTACCTCTTAAGGGATGCAGTAAAGGGAATCAAACCT
GAAGACGAGGAGAGAGCCCTTGAGGAGATGAAATCTAGAGGGATTAAGATCGTTCAGTTTTAG

1200B Codon Usage

(181 codons)
fields: [triplet] [frequency: per thousand] ([number])

UUU27.6(   5)  UCU11.0(   2)  UAU11.0(   2)  UGU5.5(   1)  
UUC11.0(   2)  UCC0.0(   0)  UAC16.6(   3)  UGC11.0(   2)  
UUA27.6(   5)  UCA0.0(   0)  UAA0.0(   0)  UGA0.0(   0)  
UUG0.0(   0)  UCG5.5(   1)  UAG5.5(   1)  UGG11.0(   2)  

CUU22.1(   4)  CCU33.1(   6)  CAU16.6(   3)  CGU0.0(   0)  
CUC5.5(   1)  CCC11.0(   2)  CAC5.5(   1)  CGC0.0(   0)  
CUA5.5(   1)  CCA11.0(   2)  CAA5.5(   1)  CGA0.0(   0)  
CUG0.0(   0)  CCG11.0(   2)  CAG11.0(   2)  CGG0.0(   0)  

AUU22.1(   4)  ACU5.5(   1)  AAU11.0(   2)  AGU0.0(   0)  
AUC22.1(   4)  ACC0.0(   0)  AAC11.0(   2)  AGC5.5(   1)  
AUA27.6(   5)  ACA16.6(   3)  AAA27.6(   5)  AGA27.6(   5)  
AUG22.1(   4)  ACG11.0(   2)  AAG49.7(   9)  AGG38.7(   7)  

GUU49.7(   9)  GCU27.6(   5)  GAU49.7(   9)  GGU16.6(   3)  
GUC11.0(   2)  GCC27.6(   5)  GAC11.0(   2)  GGC11.0(   2)  
GUA11.0(   2)  GCA22.1(   4)  GAA49.7(   9)  GGA38.7(   7)  
GUG16.6(   3)  GCG5.5(   1)  GAG55.2(  10)  GGG16.6(   3)  

1200B Potential Cloning Problems

Details (problem DNA region is in upper case, with the key on the line below):

Key:

• 3 - BclI restriction site
• 4 - BglII restriction site

atgcctgaagaagccttaatagttgttgatatgcagagagatttcatgcctggaggagct
                                                            
cttccggttcctgagggagataagataattccaaaggttaacgagtacataaggaagttt
                                                            
aaggaaaagggagctttaatagtggccacaagggattggcatcctgaaaatcacataagc
                                                            
tttagggagaggggaggcccctggcccaggcattgcgttcaaaacactcctggtgcagag
                                                            
ttcgtggtagatttaccggaagatgccgTGATCAtttcgaaagctacagagccagataaa
                            3                               
gaagcatattctggctttgaaggtacAGATCTtgcgaaaattctaagagggaatggagtt
                          4                                 
aagagagtttatatctgtggtgttgctacggagtactgcgtcagggcaacggccttagac
                                                            
gctcttaagcatgggtttgaagtctacctcttaagggatgcagtaaagggaatcaaacct
                                                            
gaagacgaggagagagcccttgaggagatgaaatctagagggattaagatcgttcagttt
                                                            
tag
   

1200B Automatically Selected Primers

Note: These were generated using Hisao's algorithm, and do not take into account possible mispriming, self-complementarity, or requirements for mutations anywhere in the sequences. The -R1 primers are for the N-terminal end, and begin with an NdeI restriction site (CATATG). The -R2 primers are for the C-terminal end, and begin with a BamHI site (GGATCC) if the target has no other BamHI sites. If the target does have a BamHI site, the -R2 primer wil begin with a BglII site (AGATCT), unless the target has no BglII sites. If the target has both sites, the design program gives up and doesn't prepend a restriction site to the -R2 primer!

1200B Translation

Note: This was generated using the standard codon usage table; UGA codons in MP/MP genes will show up as terminators ("*")

atgcctgaagaagccttaatagttgttgatatgcagagagatttcatgcctggaggagct
 M  P  E  E  A  L  I  V  V  D  M  Q  R  D  F  M  P  G  G  A 
cttccggttcctgagggagataagataattccaaaggttaacgagtacataaggaagttt
 L  P  V  P  E  G  D  K  I  I  P  K  V  N  E  Y  I  R  K  F 
aaggaaaagggagctttaatagtggccacaagggattggcatcctgaaaatcacataagc
 K  E  K  G  A  L  I  V  A  T  R  D  W  H  P  E  N  H  I  S 
tttagggagaggggaggcccctggcccaggcattgcgttcaaaacactcctggtgcagag
 F  R  E  R  G  G  P  W  P  R  H  C  V  Q  N  T  P  G  A  E 
ttcgtggtagatttaccggaagatgccgtgatcatttcgaaagctacagagccagataaa
 F  V  V  D  L  P  E  D  A  V  I  I  S  K  A  T  E  P  D  K 
gaagcatattctggctttgaaggtacagatcttgcgaaaattctaagagggaatggagtt
 E  A  Y  S  G  F  E  G  T  D  L  A  K  I  L  R  G  N  G  V 
aagagagtttatatctgtggtgttgctacggagtactgcgtcagggcaacggccttagac
 K  R  V  Y  I  C  G  V  A  T  E  Y  C  V  R  A  T  A  L  D 
gctcttaagcatgggtttgaagtctacctcttaagggatgcagtaaagggaatcaaacct
 A  L  K  H  G  F  E  V  Y  L  L  R  D  A  V  K  G  I  K  P 
gaagacgaggagagagcccttgaggagatgaaatctagagggattaagatcgttcagttt
 E  D  E  E  R  A  L  E  E  M  K  S  R  G  I  K  I  V  Q  F 
tag
 * 

1200B AA Sequence

MPEEALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIRKFKEKGALIVATRDWHPENHISFRERGGPWPRHCVQNTPGAE
FVVDLPEDAVIISKATEPDKEAYSGFEGTDLAKILRGNGVKRVYICGVATEYCVRATALDALKHGFEVYLLRDAVKGIKP
EDEERALEEMKSRGIKIVQF

1200B Protein Parameters