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This gene has the annotation "166aa long hypothetical intracellular proteinase".
The target was selected as part of Igor's sets.
Available sections:
| Experiment | Result | User | Experiment Date |
|---|---|---|---|
| Selected | Done | jmc | 2001-08-24 |
| Cloned | Done | abwaight | 2001-08-24 |
| Expression tested | Done | abwaight | 2001-08-24 |
| Solubility tested | S | abwaight | 2001-08-24 |
| Purified | Done | abwaight | 2001-08-24 |
| Crystallized | Done | abwaight | 2001-08-24 |
| Diffraction quality crystals | Done | abwaight | 2001-08-24 |
| Phasing diffraction data | Done | abwaight | 2001-08-24 |
| Traceable map | Done | abwaight | 2001-08-24 |
| Crystal structure | Done | abwaight | 2001-08-24 |
| In PDB | 1G2I | abwaight | 2001-08-24 |
| MP gene | log10(PSIBLAST E) | % identical | % coverage (of MP gene) |
|---|---|---|---|
| MP122 / MPN032 | -14.70 | 25.71 | 63.25 |
| MP541 / MPN294 | -19.15 | 18.79 | 99.40 |
GTGAAGGTACTATTCTTAACAGCGAACGAGTTTGAGGATGTGGAACTAATTTACCCTTACCATAGGCTTAAAGAAGAGGG ACATGAAGTTTACATAGCAAGCTTTGAAAGGGGAACAATTACAGGGAAACATGGATATTCAGTTAAGGTTGACTTGACTT TCGATAAAGTTAATCCCGAGGAGTTTGATGCATTAGTCCTTCCAGGAGGAAGGGCACCGGAAAGGGTAAGATTAAATGAG AAAGCTGTGAGCATAGCCAGAAAGATGTTCAGTGAAGGAAAGCCCGTTGCTAGCATATGCCATGGTCCTCAAATCTTGAT TTCTGCTGGAGTTCTAAGGGGTAGAAAGGGAACAAGCTATCCTGGGATTAAGGATGACATGATAAATGCAGGGGTTGAAT GGGTTGATGCAGAGGTAGTTGTTGATGGGAACTGGGTTAGCTCCAGGGTTCCAGCTGATCTTTATGCCTGGATGAGGGAA TTTGTAAAGTTACTTAAATGA
(167 codons) fields: [triplet] [frequency: per thousand] ([number]) UUU24.0( 4) UCU6.0( 1) UAU18.0( 3) UGU0.0( 0) UUC18.0( 3) UCC6.0( 1) UAC18.0( 3) UGC6.0( 1) UUA24.0( 4) UCA6.0( 1) UAA0.0( 0) UGA6.0( 1) UUG12.0( 2) UCG0.0( 0) UAG0.0( 0) UGG18.0( 3) CUU24.0( 4) CCU18.0( 3) CAU24.0( 4) CGU0.0( 0) CUC0.0( 0) CCC12.0( 2) CAC0.0( 0) CGC0.0( 0) CUA18.0( 3) CCA12.0( 2) CAA6.0( 1) CGA0.0( 0) CUG0.0( 0) CCG6.0( 1) CAG0.0( 0) CGG0.0( 0) AUU24.0( 4) ACU6.0( 1) AAU18.0( 3) AGU6.0( 1) AUC6.0( 1) ACC0.0( 0) AAC12.0( 2) AGC29.9( 5) AUA24.0( 4) ACA24.0( 4) AAA29.9( 5) AGA18.0( 3) AUG18.0( 3) ACG0.0( 0) AAG41.9( 7) AGG41.9( 7) GUU71.9( 12) GCU24.0( 4) GAU41.9( 7) GGU12.0( 2) GUC6.0( 1) GCC12.0( 2) GAC12.0( 2) GGC0.0( 0) GUA24.0( 4) GCA29.9( 5) GAA47.9( 8) GGA47.9( 8) GUG18.0( 3) GCG6.0( 1) GAG41.9( 7) GGG24.0( 4)
Details (problem DNA region is in upper case, with the key on the line below):
Key:
gtgaaggtactattcttaacagcgaacgagtttgaggatgtggaactaatttacccttac
cataggcttaaagaagagggacatgaagtttacatagcaagctttgaaaggggaacaatt
acagggaaacatggatattcagttaaggttgacttgactttcgataaagttaatcccgag
gagtttgatgcattagtccttccaggaggaagggcaccggaaagggtaagattaaatgag
aaagctgtgagcatagccagaaagatgttcagtgaaggaaagcccgttgctagCATATGc
1
catggtcctcaaatcttgatttctgctggagttctaaggggtagaaagggaacaagctat
cctgggattaaggatgacatgataaatgcaggggttgaatgggttgatgcagaggtagtt
gttgatgggaactgggttagctccagggttccagctgatctttatgcctggatgagggaa
tttgtaaagttacttaaatga
Note: These were generated using Hisao's algorithm, and do not take into account possible mispriming, self-complementarity, or requirements for mutations anywhere in the sequences. The -R1 primers are for the N-terminal end, and begin with an NdeI restriction site (CATATG). The -R2 primers are for the C-terminal end, and begin with a BamHI site (GGATCC) if the target has no other BamHI sites. If the target does have a BamHI site, the -R2 primer wil begin with a BglII site (AGATCT), unless the target has no BglII sites. If the target has both sites, the design program gives up and doesn't prepend a restriction site to the -R2 primer!
Predicted Tm was obtained using the Tm determination tool at the Virtual Genome Center.
| Primer ID | Predicted Tm | sequence |
|---|---|---|
| 1022B-R1 | 62.90 | CATATGAAGGTACTATTCTTAACAGCGAACG |
| 1022B-R2 | 61.10 | GGATCCTTATCATTTAAACTTTACAAATTCCCTCATC |
| 1022B-R3 | 61.40 | GGATCCTTATTTAAGTAACTTTACAAATTCCCTCATCC |
| 1022B-R10 | 64.30 | GGCGGTGGTGGCGGCATGAAGGTACTATTCTTAACAGCGAACGA |
| 1022B-R11 | 63.20 | GTTCTTCTCCTTTGCGCCCCTATTTAAGTAACTTTACAAATTCCCTCATCCA |
Note: This was generated using the standard codon usage table; UGA codons in MP/MP genes will show up as terminators ("*")
gtgaaggtactattcttaacagcgaacgagtttgaggatgtggaactaatttacccttac V K V L F L T A N E F E D V E L I Y P Y cataggcttaaagaagagggacatgaagtttacatagcaagctttgaaaggggaacaatt H R L K E E G H E V Y I A S F E R G T I acagggaaacatggatattcagttaaggttgacttgactttcgataaagttaatcccgag T G K H G Y S V K V D L T F D K V N P E gagtttgatgcattagtccttccaggaggaagggcaccggaaagggtaagattaaatgag E F D A L V L P G G R A P E R V R L N E aaagctgtgagcatagccagaaagatgttcagtgaaggaaagcccgttgctagcatatgc K A V S I A R K M F S E G K P V A S I C catggtcctcaaatcttgatttctgctggagttctaaggggtagaaagggaacaagctat H G P Q I L I S A G V L R G R K G T S Y cctgggattaaggatgacatgataaatgcaggggttgaatgggttgatgcagaggtagtt P G I K D D M I N A G V E W V D A E V V gttgatgggaactgggttagctccagggttccagctgatctttatgcctggatgagggaa V D G N W V S S R V P A D L Y A W M R E tttgtaaagttacttaaatga F V K L L K *
MKVLFLTANEFEDVELIYPYHRLKEEGHEVYIASFERGTITGKHGYSVKVDLTFDKVNPEEFDALVLPGGRAPERVRLNE KAVSIARKMFSEGKPVASICHGPQILISAGVLRGRKGTSYPGIKDDMINAGVEWVDAEVVVDGNWVSSRVPADLYAWMRE FVKLLK
This information was obtained using the Protein Parameters tool on the ExPASy Molecular Biology Server.
Number of amino acids: 166
Molecular weight: 18624.4
Theoretical pI: 6.11
Amino acid composition:
Ala (A) 12 7.2%
Arg (R) 10 6.0%
Asn (N) 5 3.0%
Asp (D) 9 5.4%
Cys (C) 1 0.6%
Gln (Q) 1 0.6%
Glu (E) 15 9.0%
Gly (G) 14 8.4%
His (H) 4 2.4%
Ile (I) 9 5.4%
Leu (L) 13 7.8%
Lys (K) 12 7.2%
Met (M) 4 2.4%
Phe (F) 7 4.2%
Pro (P) 8 4.8%
Ser (S) 9 5.4%
Thr (T) 5 3.0%
Trp (W) 3 1.8%
Tyr (Y) 6 3.6%
Val (V) 19 11.4%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 843
Hydrogen H 1321
Nitrogen N 225
Oxygen O 241
Sulfur S 5
Formula: C843H1321N225O241S5
Total number of atoms: 2635
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 24900 25200 25050 24750 24000
Abs 0.1% (=1 g/l) 1.337 1.353 1.345 1.329 1.289
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 24900 25200 25050 24750 24000
Abs 0.1% (=1 g/l) 1.337 1.353 1.345 1.329 1.289
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 23.24
This classifies the protein as stable.
Aliphatic index: 92.11
Grand average of hydropathicity (GRAVY): -0.170