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This gene has the annotation "orf, hypothetical protein".
The target was selected as part of pre-BSGC set.
Available sections:
| Experiment | Result | User | Experiment Date |
|---|---|---|---|
| Selected | Done | jmc | 2003-09-26 |
| Cloned | Done | dhshin | 2003-10-01 |
| Expression tested | Done | dhshin | 2003-10-01 |
| Solubility tested | S | dhshin | 2003-10-01 |
| Purified | Done | dhshin | 2003-10-01 |
| Crystallized | Done | dhshin | 2003-10-01 |
| Diffraction quality crystals | Done | dhshin | 2003-10-01 |
| Native diffraction data | Done | dhshin | 2003-10-01 |
| Phasing diffraction data | Done | dhshin | 2003-10-01 |
| Traceable map | Done | dhshin | 2003-10-01 |
| Crystal structure | Done | dhshin | 2004-01-29 |
| In PDB | 1S7D | dhshin | 2004-01-29 |
TTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCA CTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAA CGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAG AAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGAT GATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACA AAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAA TATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCA ATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGA TGTCTCATTGA
(217 codons) fields: [triplet] [frequency: per thousand] ([number]) UUU36.9( 8) UCU13.8( 3) UAU46.1( 10) UGU9.2( 2) UUC9.2( 2) UCC4.6( 1) UAC18.4( 4) UGC0.0( 0) UUA27.6( 6) UCA18.4( 4) UAA0.0( 0) UGA4.6( 1) UUG9.2( 2) UCG9.2( 2) UAG0.0( 0) UGG13.8( 3) CUU9.2( 2) CCU18.4( 4) CAU27.6( 6) CGU9.2( 2) CUC9.2( 2) CCC9.2( 2) CAC18.4( 4) CGC0.0( 0) CUA0.0( 0) CCA13.8( 3) CAA27.6( 6) CGA9.2( 2) CUG9.2( 2) CCG0.0( 0) CAG13.8( 3) CGG0.0( 0) AUU36.9( 8) ACU4.6( 1) AAU27.6( 6) AGU13.8( 3) AUC4.6( 1) ACC4.6( 1) AAC9.2( 2) AGC18.4( 4) AUA13.8( 3) ACA23.0( 5) AAA78.3( 17) AGA4.6( 1) AUG23.0( 5) ACG9.2( 2) AAG9.2( 2) AGG0.0( 0) GUU27.6( 6) GCU27.6( 6) GAU55.3( 12) GGU23.0( 5) GUC23.0( 5) GCC13.8( 3) GAC13.8( 3) GGC27.6( 6) GUA4.6( 1) GCA13.8( 3) GAA50.7( 11) GGA9.2( 2) GUG4.6( 1) GCG9.2( 2) GAG18.4( 4) GGG0.0( 0)
Note: These were generated using Hisao's algorithm, and do not take into account possible mispriming, self-complementarity, or requirements for mutations anywhere in the sequences. The -R1 primers are for the N-terminal end, and begin with an NdeI restriction site (CATATG). The -R2 primers are for the C-terminal end, and begin with a BamHI site (GGATCC) if the target has no other BamHI sites. If the target does have a BamHI site, the -R2 primer wil begin with a BglII site (AGATCT), unless the target has no BglII sites. If the target has both sites, the design program gives up and doesn't prepend a restriction site to the -R2 primer!
Predicted Tm was obtained using the Tm determination tool at the Virtual Genome Center.
| Primer ID | Predicted Tm | sequence |
|---|---|---|
| 1387B-R10 | 63.40 | GGCGGTGGTGGCGGCATGGCGATTCGTCTTTACAAACTG |
| 1387B-R11 | 64.00 | GTTCTTCTCCTTTGCGCCCCTAATGAGACATCATTTCCTCGACCA |
Note: This was generated using the standard codon usage table; UGA codons in MP/MP genes will show up as terminators ("*")
ttggcgattcgtctttacaaactggctgttgctttaggtgtctttattgttagcgctcct L A I R L Y K L A V A L G V F I V S A P gccttttcgcatggtcatcactcacacggcaaacccttaacagaggtcgaacaaaaagct A F S H G H H S H G K P L T E V E Q K A gctaatggtgtttttgatgatgccaatgtacaaaaccgaacgctcagtgactgggatgga A N G V F D D A N V Q N R T L S D W D G gtctggcaatccgtttatcctttactgcaaagtggcaaacttgaccccgtctttcagaag V W Q S V Y P L L Q S G K L D P V F Q K aaagcggatgcagataaaactaaaacatttgctgaaattaaagattattatcacaaaggt K A D A D K T K T F A E I K D Y Y H K G tatgcaacagatatcgagatgattggcattgaggacggcattgttgaattccatagaaat Y A T D I E M I G I E D G I V E F H R N aatgaaacaacatcgtgtaaatatgattacgatggatacaaaatactcacctataaatca N E T T S C K Y D Y D G Y K I L T Y K S ggcaagaaaggcgttcgttacttattcgaatgtaaagatcctgaaagcaaagcccctaaa G K K G V R Y L F E C K D P E S K A P K tatatacaatttagcgatcatataattgcaccacgaaaatcatctcattttcacattttt Y I Q F S D H I I A P R K S S H F H I F atgggtaatgattcacagcaatctttattaaatgaaatggaaaactggccaacgtattat M G N D S Q Q S L L N E M E N W P T Y Y ccatatcagttgagtagcgaagaagtggtcgaggaaatgatgtctcattga P Y Q L S S E E V V E E M M S H *
MAIRLYKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGVFDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQK KADADKTKTFAEIKDYYHKGYATDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH
This information was obtained using the Protein Parameters tool on the ExPASy Molecular Biology Server.
Number of amino acids: 216
Molecular weight: 24761.8
Theoretical pI: 5.91
Amino acid composition:
Ala (A) 14 6.5%
Arg (R) 5 2.3%
Asn (N) 8 3.7%
Asp (D) 15 6.9%
Cys (C) 2 0.9%
Gln (Q) 9 4.2%
Glu (E) 15 6.9%
Gly (G) 13 6.0%
His (H) 10 4.6%
Ile (I) 12 5.6%
Leu (L) 13 6.0%
Lys (K) 19 8.8%
Met (M) 6 2.8%
Phe (F) 10 4.6%
Pro (P) 9 4.2%
Ser (S) 17 7.9%
Thr (T) 9 4.2%
Trp (W) 3 1.4%
Tyr (Y) 14 6.5%
Val (V) 13 6.0%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 1116
Hydrogen H 1682
Nitrogen N 290
Oxygen O 334
Sulfur S 8
Formula: C1116H1682N290O334S8
Total number of atoms: 3430
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 36645 36527 35930 35110 33720
Abs 0.1% (=1 g/l) 1.480 1.475 1.451 1.418 1.362
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 36500 36400 35810 34990 33600
Abs 0.1% (=1 g/l) 1.474 1.470 1.446 1.413 1.357
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.83
This classifies the protein as unstable.
Aliphatic index: 69.07
Grand average of hydropathicity (GRAVY): -0.583