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This gene has the annotation "MG427 homolog, from M. genitalium".
The target was selected as part of Igor's sets.
Available sections:
| Experiment | Result | User | Experiment Date |
|---|---|---|---|
| Selected | Done | igrigoriev | 2001-08-24 |
| Cloned | Done | abwaight | 2001-08-24 |
| Expression tested | Done | abwaight | 2001-08-24 |
| Solubility tested | S | abwaight | 2001-08-24 |
| Purified | Done | abwaight | 2001-08-24 |
| Crystallized | Done | abwaight | 2001-08-24 |
| Diffraction quality crystals | Done | abwaight | 2001-08-24 |
| Phasing diffraction data | Done | abwaight | 2001-08-24 |
| Traceable map | Done | abwaight | 2001-08-24 |
| Crystal structure | Done | abwaight | 2001-08-24 |
| In PDB | 1LQL | dhshin | 2002-11-01 |
| MP gene | log10(PSIBLAST E) | % identical | % coverage (of MP gene) |
|---|---|---|---|
| MP174 / MPN668 | -18.70 | 16.91 | 96.45 |
| MP217 / MPN625 | -27.30 | 100.00 | 100.00 |
ATGGACAAAAAGTACGACATCACTGCTGTTTTAAACGAAGACAGCTCCATGACTGCCATCAGTGATCAGTTTCAAATTAC ACTCGATGCCCGTCCCAAGCACACCGCTAAGGGTTTTGGTCCTTTAGCTGCTCTGCTCTCTGGTTTAGCTGCTTGTGAAT TGGCAACCGCTAACTTGATGGCACCAGCTAAAATGATTACCATCAATAAACTGTTGATGAATGTCACTGGTTCACGCTCA ACCAACCCCACCGATGGTTATTTTGGTTTAAGGGAAATTAACCTGCACTGGGAAATCCACTCCCCTAACAGTGAAACGGA AATCAAGGAGTTCATTGACTTTGTTTCCAAACGCTGCCCAGCGCACAATACGCTCCAAGGTGTGAGTCAACTCAAGATTA ACGTTAATGTAACTTTAGTGCATTAA
(142 codons) fields: [triplet] [frequency: per thousand] ([number]) UUU28.2( 4) UCU7.0( 1) UAU7.0( 1) UGU7.0( 1) UUC7.0( 1) UCC21.1( 3) UAC7.0( 1) UGC7.0( 1) UUA35.2( 5) UCA14.1( 2) UAA7.0( 1) UGA0.0( 0) UUG21.1( 3) UCG0.0( 0) UAG0.0( 0) UGG7.0( 1) CUU0.0( 0) CCU14.1( 2) CAU7.0( 1) CGU7.0( 1) CUC28.2( 4) CCC14.1( 2) CAC28.2( 4) CGC14.1( 2) CUA0.0( 0) CCA14.1( 2) CAA21.1( 3) CGA0.0( 0) CUG21.1( 3) CCG0.0( 0) CAG7.0( 1) CGG0.0( 0) AUU35.2( 5) ACU28.2( 4) AAU28.2( 4) AGU21.1( 3) AUC35.2( 5) ACC35.2( 5) AAC42.3( 6) AGC7.0( 1) AUA0.0( 0) ACA7.0( 1) AAA28.2( 4) AGA0.0( 0) AUG35.2( 5) ACG14.1( 2) AAG35.2( 5) AGG7.0( 1) GUU21.1( 3) GCU56.3( 8) GAU21.1( 3) GGU49.3( 7) GUC7.0( 1) GCC14.1( 2) GAC28.2( 4) GGC0.0( 0) GUA7.0( 1) GCA14.1( 2) GAA42.3( 6) GGA0.0( 0) GUG14.1( 2) GCG7.0( 1) GAG7.0( 1) GGG0.0( 0)
Details (problem DNA region is in upper case, with the key on the line below):
Key:
atggacaaaaagtacgacatcactgctgttttaaacgaagacagctccatgactgccatc
agTGATCAgtttcaaattacactcgatgcccgtcccaagcacaccgctaagggttttggt
3
cctttagctgctctgctctctggtttagctgcttgtgaattggcaaccgctaacttgatg
gcaccagctaaaatgattaccatcaataaactgttgatgaatgtcactggttcacgctca
accaaccccaccgatggttattttggtttaagggaaattaacctgcactgggaaatccac
tcccctaacagtgaaacggaaatcaaggagttcattgactttgtttccaaacgctgccca
gcgcacaatacgctccaaggtgtgagtcaactcaagattaacgttaatgtaactttagtg
cattaa
Note: These were generated using Hisao's algorithm, and do not take into account possible mispriming, self-complementarity, or requirements for mutations anywhere in the sequences. The -R1 primers are for the N-terminal end, and begin with an NdeI restriction site (CATATG). The -R2 primers are for the C-terminal end, and begin with a BamHI site (GGATCC) if the target has no other BamHI sites. If the target does have a BamHI site, the -R2 primer wil begin with a BglII site (AGATCT), unless the target has no BglII sites. If the target has both sites, the design program gives up and doesn't prepend a restriction site to the -R2 primer!
Predicted Tm was obtained using the Tm determination tool at the Virtual Genome Center.
| Primer ID | Predicted Tm | sequence |
|---|---|---|
| 1038B-R1 | 61.40 | CATATGGACAAAAAGTACGACATCACTG |
| 1038B-R2 | 60.30 | GGATCCTTAATGCACTAAAGTTACATTAACGTTAATCT |
| 1038B-R10 | 64.50 | GGCGGTGGTGGCGGCATGGACAAAAAGTACGACATCACTGC |
| 1038B-R11 | 63.20 | GTTCTTCTCCTTTGCGCCCCTAATGCACTAAAGTTACATTAACGTTAATCTTGA |
Note: This was generated using the standard codon usage table; UGA codons in MP/MP genes will show up as terminators ("*")
atggacaaaaagtacgacatcactgctgttttaaacgaagacagctccatgactgccatc M D K K Y D I T A V L N E D S S M T A I agtgatcagtttcaaattacactcgatgcccgtcccaagcacaccgctaagggttttggt S D Q F Q I T L D A R P K H T A K G F G cctttagctgctctgctctctggtttagctgcttgtgaattggcaaccgctaacttgatg P L A A L L S G L A A C E L A T A N L M gcaccagctaaaatgattaccatcaataaactgttgatgaatgtcactggttcacgctca A P A K M I T I N K L L M N V T G S R S accaaccccaccgatggttattttggtttaagggaaattaacctgcactgggaaatccac T N P T D G Y F G L R E I N L H W E I H tcccctaacagtgaaacggaaatcaaggagttcattgactttgtttccaaacgctgccca S P N S E T E I K E F I D F V S K R C P gcgcacaatacgctccaaggtgtgagtcaactcaagattaacgttaatgtaactttagtg A H N T L Q G V S Q L K I N V N V T L V cattaa H *
MDKKYDITAVLNEDSSMTAISDQFQITLDARPKHTAKGFGPLAALLSGLAACELATANLMAPAKMITINKLLMNVTGSRS TNPTDGYFGLREINLHWEIHSPNSETEIKEFIDFVSKRCPAHNTLQGVSQLKINVNVTLVH
This information was obtained using the Protein Parameters tool on the ExPASy Molecular Biology Server.
Number of amino acids: 141
Molecular weight: 15468.7
Theoretical pI: 6.50
Amino acid composition:
Ala (A) 13 9.2%
Arg (R) 4 2.8%
Asn (N) 10 7.1%
Asp (D) 7 5.0%
Cys (C) 2 1.4%
Gln (Q) 4 2.8%
Glu (E) 7 5.0%
Gly (G) 7 5.0%
His (H) 5 3.5%
Ile (I) 10 7.1%
Leu (L) 15 10.6%
Lys (K) 9 6.4%
Met (M) 5 3.5%
Phe (F) 5 3.5%
Pro (P) 6 4.3%
Ser (S) 10 7.1%
Thr (T) 12 8.5%
Trp (W) 1 0.7%
Tyr (Y) 2 1.4%
Val (V) 7 5.0%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 14
Total number of positively charged residues (Arg + Lys): 13
Atomic composition:
Carbon C 682
Hydrogen H 1097
Nitrogen N 187
Oxygen O 208
Sulfur S 7
Formula: C682H1097N187O208S7
Total number of atoms: 2181
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 8445 8527 8470 8370 8120
Abs 0.1% (=1 g/l) 0.546 0.551 0.548 0.541 0.525
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 8300 8400 8350 8250 8000
Abs 0.1% (=1 g/l) 0.537 0.543 0.540 0.533 0.517
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.35
This classifies the protein as unstable.
Aliphatic index: 92.77
Grand average of hydropathicity (GRAVY): -0.114