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This gene has the annotation "fibrillarin (fib)".
The target was selected as part of Igor's sets.
Available sections:
| Experiment | Result | User | Experiment Date |
|---|---|---|---|
| Selected | Done | jmc | 2001-08-24 |
| Cloned | Done | abwaight | 2001-08-24 |
| Expression tested | Done | abwaight | 2001-08-24 |
| Solubility tested | S | abwaight | 2001-08-24 |
| Purified | Done | abwaight | 2001-08-24 |
| Crystallized | Done | abwaight | 2001-08-24 |
| Diffraction quality crystals | Done | abwaight | 2001-08-24 |
| Phasing diffraction data | Done | abwaight | 2001-08-24 |
| Traceable map | Done | abwaight | 2001-08-24 |
| Crystal structure | Done | abwaight | 2001-08-24 |
| In PDB | 1FBN | abwaight | 2001-08-24 |
| MP gene | log10(PSIBLAST E) | % identical | % coverage (of MP gene) |
|---|---|---|---|
| MP474 / MPN362 | -8.70 | 16.67 | 39.13 |
| MP485 / MPN351 | -5.00 | 15.73 | 38.70 |
| MP521 / MPN315 | -5.70 | 16.00 | 43.48 |
ATGGAAGACATTAAAATCAAAGAGATTTTTGAAAACATCTATGAAGTTGATTTAGGAGATGGCTTAAAAAGAATAGCAAC AAAATCTATTGTTAAGGGAAAAAAAGTATATGATGAAAAAATAATAAAAATTGGAGATGAAGAGTATAGAATTTGGAATC CAAATAAAAGTAAGTTGGCAGCTGCAATAATTAAAGGTTTAAAGGTTATGCCAATAAAAAGAGATTCAAAAATCTTATAC TTAGGAGCTTCAGCTGGAACTACACCATCTCACGTTGCAGATATTGCTGATAAAGGCATTGTATATGCCATAGAGTATGC ACCAAGGATTATGAGAGAGCTTTTAGATGCATGTGCTGAGAGGGAAAACATAATCCCAATTTTAGGAGATGCAAATAAAC CTCAAGAATATGCAAATATTGTTGAAAAGGTAGATGTTATCTATGAGGATGTTGCTCAGCCAAATCAAGCGGAGATTTTA ATTAAAAATGCTAAGTGGTTTTTAAAGAAAGGCGGTTATGGAATGATAGCAATAAAGGCAAGGAGTATAGATGTTACAAA AGACCCAAAAGAGATTTTTAAAGAACAAAAAGAAATTTTAGAGGCAGGAGGTTTTAAAATAGTTGATGAGGTAGATATTG AGCCGTTTGAAAAAGACCACGTCATGTTTGTAGGTATTTGGGAGGGAAAATAA
(231 codons) fields: [triplet] [frequency: per thousand] ([number]) UUU26.0( 6) UCU8.7( 2) UAU34.6( 8) UGU4.3( 1) UUC0.0( 0) UCC0.0( 0) UAC4.3( 1) UGC0.0( 0) UUA43.3( 10) UCA8.7( 2) UAA4.3( 1) UGA0.0( 0) UUG4.3( 1) UCG0.0( 0) UAG0.0( 0) UGG13.0( 3) CUU4.3( 1) CCU4.3( 1) CAU0.0( 0) CGU0.0( 0) CUC0.0( 0) CCC0.0( 0) CAC8.7( 2) CGC0.0( 0) CUA0.0( 0) CCA30.3( 7) CAA13.0( 3) CGA0.0( 0) CUG0.0( 0) CCG4.3( 1) CAG4.3( 1) CGG0.0( 0) AUU73.6( 17) ACU4.3( 1) AAU26.0( 6) AGU8.7( 2) AUC21.6( 5) ACC0.0( 0) AAC8.7( 2) AGC0.0( 0) AUA47.6( 11) ACA13.0( 3) AAA99.6( 23) AGA17.3( 4) AUG21.6( 5) ACG0.0( 0) AAG30.3( 7) AGG13.0( 3) GUU39.0( 9) GCU30.3( 7) GAU60.6( 14) GGU17.3( 4) GUC4.3( 1) GCC4.3( 1) GAC13.0( 3) GGC13.0( 3) GUA21.6( 5) GCA47.6( 11) GAA47.6( 11) GGA39.0( 9) GUG0.0( 0) GCG4.3( 1) GAG51.9( 12) GGG0.0( 0)
Note: These were generated using Hisao's algorithm, and do not take into account possible mispriming, self-complementarity, or requirements for mutations anywhere in the sequences. The -R1 primers are for the N-terminal end, and begin with an NdeI restriction site (CATATG). The -R2 primers are for the C-terminal end, and begin with a BamHI site (GGATCC) if the target has no other BamHI sites. If the target does have a BamHI site, the -R2 primer wil begin with a BglII site (AGATCT), unless the target has no BglII sites. If the target has both sites, the design program gives up and doesn't prepend a restriction site to the -R2 primer!
Predicted Tm was obtained using the Tm determination tool at the Virtual Genome Center.
| Primer ID | Predicted Tm | sequence |
|---|---|---|
| 1055B-R1 | 60.90 | CATATGGAAGACATTAAAATCAAAGAGATTTT |
| 1055B-R2 | 60.90 | GGATCCTTATTTTCCCTCCCAAATACCTACA |
| 1055B-R10 | 63.70 | GGCGGTGGTGGCGGCATGGAAGACATTAAAATCAAAGAGATTTTTG |
| 1055B-R11 | 64.50 | GTTCTTCTCCTTTGCGCCCCTATTTTCCCTCCCAAATACCTACAAACA |
Note: This was generated using the standard codon usage table; UGA codons in MP/MP genes will show up as terminators ("*")
atggaagacattaaaatcaaagagatttttgaaaacatctatgaagttgatttaggagat M E D I K I K E I F E N I Y E V D L G D ggcttaaaaagaatagcaacaaaatctattgttaagggaaaaaaagtatatgatgaaaaa G L K R I A T K S I V K G K K V Y D E K ataataaaaattggagatgaagagtatagaatttggaatccaaataaaagtaagttggca I I K I G D E E Y R I W N P N K S K L A gctgcaataattaaaggtttaaaggttatgccaataaaaagagattcaaaaatcttatac A A I I K G L K V M P I K R D S K I L Y ttaggagcttcagctggaactacaccatctcacgttgcagatattgctgataaaggcatt L G A S A G T T P S H V A D I A D K G I gtatatgccatagagtatgcaccaaggattatgagagagcttttagatgcatgtgctgag V Y A I E Y A P R I M R E L L D A C A E agggaaaacataatcccaattttaggagatgcaaataaacctcaagaatatgcaaatatt R E N I I P I L G D A N K P Q E Y A N I gttgaaaaggtagatgttatctatgaggatgttgctcagccaaatcaagcggagatttta V E K V D V I Y E D V A Q P N Q A E I L attaaaaatgctaagtggtttttaaagaaaggcggttatggaatgatagcaataaaggca I K N A K W F L K K G G Y G M I A I K A aggagtatagatgttacaaaagacccaaaagagatttttaaagaacaaaaagaaatttta R S I D V T K D P K E I F K E Q K E I L gaggcaggaggttttaaaatagttgatgaggtagatattgagccgtttgaaaaagaccac E A G G F K I V D E V D I E P F E K D H gtcatgtttgtaggtatttgggagggaaaataa V M F V G I W E G K *
MEDIKIKEIFENIYEVDLGDGLKRIATKSIVKGKKVYDEKIIKIGDEEYRIWNPNKSKLAAAIIKGLKVMPIKRDSKILY LGASAGTTPSHVADIADKGIVYAIEYAPRIMRELLDACAERENIIPILGDANKPQEYANIVEKVDVIYEDVAQPNQAEIL IKNAKWFLKKGGYGMIAIKARSIDVTKDPKEIFKEQKEILEAGGFKIVDEVDIEPFEKDHVMFVGIWEGK
This information was obtained using the Protein Parameters tool on the ExPASy Molecular Biology Server.
Number of amino acids: 230
Molecular weight: 25966.2
Theoretical pI: 5.67
Amino acid composition:
Ala (A) 20 8.7%
Arg (R) 7 3.0%
Asn (N) 8 3.5%
Asp (D) 17 7.4%
Cys (C) 1 0.4%
Gln (Q) 4 1.7%
Glu (E) 23 10.0%
Gly (G) 16 7.0%
His (H) 2 0.9%
Ile (I) 33 14.3%
Leu (L) 12 5.2%
Lys (K) 30 13.0%
Met (M) 5 2.2%
Phe (F) 6 2.6%
Pro (P) 9 3.9%
Ser (S) 6 2.6%
Thr (T) 4 1.7%
Trp (W) 3 1.3%
Tyr (Y) 9 3.9%
Val (V) 15 6.5%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 1181
Hydrogen H 1900
Nitrogen N 300
Oxygen O 342
Sulfur S 6
Formula: C1181H1900N300O342S6
Total number of atoms: 3729
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 29250 29400 29085 28590 27600
Abs 0.1% (=1 g/l) 1.126 1.132 1.120 1.101 1.063
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 29250 29400 29085 28590 27600
Abs 0.1% (=1 g/l) 1.126 1.132 1.120 1.101 1.063
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.77
This classifies the protein as stable.
Aliphatic index: 103.91
Grand average of hydropathicity (GRAVY): -0.251