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This gene has the annotation "putative carbonic anhdrase (EC 4.2.1.1)".
The target was selected as part of CompIII targets.
Available sections:
| Experiment | Result | User | Experiment Date |
|---|---|---|---|
| Selected | Done | jmc | 2004-04-26 |
| Cloned | Done | dhshin | 2004-04-27 |
| Expression tested | Done | dhshin | 2004-04-27 |
| Solubility tested | S | dhshin | 2004-04-27 |
| Purified | Done | dhshin | 2004-04-27 |
| Crystallized | Done | dhshin | 2004-04-27 |
| Diffraction quality crystals | Done | dhshin | 2004-04-27 |
| Native diffraction data | Done | dhshin | 2004-04-27 |
| Phasing diffraction data | Done | dhshin | 2004-04-27 |
| Traceable map | Done | dhshin | 2004-04-27 |
| Crystal structure | Done | dhshin | 2004-04-27 |
| In PDB | 1T75 | dhshin | 2004-05-10 |
ATGAAAGACATAGATACACTCATCAGCAACAATGCACTATGGTCAAAAATGCTGGTGGAAGAGGATCCCGGGTTTTTTGA GAAACTGGCACAAGCGCAAAAACCGCGCTTTCTATGGATTGGATGTTCCGACAGTCGCGTTCCTGCAGAACGTTTAACCG GTCTTGAGCCGGGCGAACTCTTTGTTCACCGTAATGTTGCTAACCTGGTCATTCACACTGACCTGAACTGCCTTTCCGTG GTTCAGTATGCAGTGGATGTACTCGAAGTTGAACACATTATTATCTGTGGCCACTACGGTTGCGGCGGCGTACAAGCCGC AGTTGAAAACCCGGAACTGGGGCTTATCAACAACTGGCTGCTGCATATCCGCGATATCTGGTTCAAACATAGCTCATTGC TCGGCGAAATGCCGCAAGAGCGCCGTCTGGATACCTTGTGTGAACTGAACGTCATGGAACAGGTGTATAACCTGGGCCAC TCCACCATTATGCAATCAGCGTGGAAACGCGGGCAGAAAGTTACCATTCACGGCTGGGCCTACGGCATTCACGACGGCTT GCTGCGTGATCTGGATGTTACCGCCACCAACCGCGAAACCCTTGAGCAACGTTACCGTCACGGGATTTCCAACCTCAAGC TGAAACACGCCAACCACAAATAA
(221 codons) fields: [triplet] [frequency: per thousand] ([number]) UUU18.1( 4) UCU0.0( 0) UAU9.0( 2) UGU13.6( 3) UUC4.5( 1) UCC18.1( 4) UAC13.6( 3) UGC9.0( 2) UUA4.5( 1) UCA13.6( 3) UAA4.5( 1) UGA0.0( 0) UUG13.6( 3) UCG0.0( 0) UAG0.0( 0) UGG27.1( 6) CUU18.1( 4) CCU4.5( 1) CAU9.0( 2) CGU27.1( 6) CUC22.6( 5) CCC4.5( 1) CAC45.2( 10) CGC27.1( 6) CUA9.0( 2) CCA0.0( 0) CAA27.1( 6) CGA0.0( 0) CUG58.8( 13) CCG18.1( 4) CAG13.6( 3) CGG0.0( 0) AUU36.2( 8) ACU4.5( 1) AAU9.0( 2) AGU4.5( 1) AUC22.6( 5) ACC31.7( 7) AAC49.8( 11) AGC9.0( 2) AUA4.5( 1) ACA4.5( 1) AAA40.7( 9) AGA0.0( 0) AUG22.6( 5) ACG0.0( 0) AAG4.5( 1) AGG0.0( 0) GUU36.2( 8) GCU4.5( 1) GAU31.7( 7) GGU9.0( 2) GUC9.0( 2) GCC18.1( 4) GAC18.1( 4) GGC40.7( 9) GUA9.0( 2) GCA22.6( 5) GAA49.8( 11) GGA4.5( 1) GUG18.1( 4) GCG9.0( 2) GAG22.6( 5) GGG18.1( 4)
Details (problem DNA region is in upper case, with the key on the line below):
Key:
atgaaagacatagatacactcatcagcaacaatgcactatggtcaaaaatgctggtggaa
gaGGATCCcgggttttttgagaaactggcacaagcgcaaaaaccgcgctttctatggatt
2
ggatgttccgacagtcgcgttcctgcagaacgtttaaccggtcttgagccgggcgaactc
tttgttcaccgtaatgttgctaacctggtcattcacactgacctgaactgcctttccgtg
gttcagtatgcagtggatgtactcgaagttgaacacattattatctgtggccactacggt
tgcggcggcgtacaagccgcagttgaaaacccggaactggggcttatcaacaactggctg
ctgcatatccgcgatatctggttcaaacatagctcattgctcggcgaaatgccgcaagag
cgccgtctggataccttgtgtgaactgaacgtcatggaacaggtgtataacctgggccac
tccaccattatgcaatcagcgtggaaacgcgggcagaaagttaccattcacggctgggcc
tacggcattcacgacggcttgctgcgtgatctggatgttaccgccaccaaccgcgaaacc
cttgagcaacgttaccgtcacgggatttccaacctcaagctgaaacacgccaaccacaaa
taa
Note: These were generated using Hisao's algorithm, and do not take into account possible mispriming, self-complementarity, or requirements for mutations anywhere in the sequences. The -R1 primers are for the N-terminal end, and begin with an NdeI restriction site (CATATG). The -R2 primers are for the C-terminal end, and begin with a BamHI site (GGATCC) if the target has no other BamHI sites. If the target does have a BamHI site, the -R2 primer wil begin with a BglII site (AGATCT), unless the target has no BglII sites. If the target has both sites, the design program gives up and doesn't prepend a restriction site to the -R2 primer!
Predicted Tm was obtained using the Tm determination tool at the Virtual Genome Center.
| Primer ID | Predicted Tm | sequence |
|---|---|---|
| 1944B-R10 | 63.00 | GGCGGTGGTGGCGGCATGATGAAAGACATAGATACACTCATCAGCAAC |
| 1944B-R11 | 64.40 | GTTCTTCTCCTTTGCGCCCCTATTATTTGTGGTTGGCGTGTTTCA |
Note: This was generated using the standard codon usage table; UGA codons in MP/MP genes will show up as terminators ("*")
atgaaagacatagatacactcatcagcaacaatgcactatggtcaaaaatgctggtggaa M K D I D T L I S N N A L W S K M L V E gaggatcccgggttttttgagaaactggcacaagcgcaaaaaccgcgctttctatggatt E D P G F F E K L A Q A Q K P R F L W I ggatgttccgacagtcgcgttcctgcagaacgtttaaccggtcttgagccgggcgaactc G C S D S R V P A E R L T G L E P G E L tttgttcaccgtaatgttgctaacctggtcattcacactgacctgaactgcctttccgtg F V H R N V A N L V I H T D L N C L S V gttcagtatgcagtggatgtactcgaagttgaacacattattatctgtggccactacggt V Q Y A V D V L E V E H I I I C G H Y G tgcggcggcgtacaagccgcagttgaaaacccggaactggggcttatcaacaactggctg C G G V Q A A V E N P E L G L I N N W L ctgcatatccgcgatatctggttcaaacatagctcattgctcggcgaaatgccgcaagag L H I R D I W F K H S S L L G E M P Q E cgccgtctggataccttgtgtgaactgaacgtcatggaacaggtgtataacctgggccac R R L D T L C E L N V M E Q V Y N L G H tccaccattatgcaatcagcgtggaaacgcgggcagaaagttaccattcacggctgggcc S T I M Q S A W K R G Q K V T I H G W A tacggcattcacgacggcttgctgcgtgatctggatgttaccgccaccaaccgcgaaacc Y G I H D G L L R D L D V T A T N R E T cttgagcaacgttaccgtcacgggatttccaacctcaagctgaaacacgccaaccacaaa L E Q R Y R H G I S N L K L K H A N H K taa *
MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSDSRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSV VQYAVDVLEVEHIIICGHYGCGGVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHANHK
This information was obtained using the Protein Parameters tool on the ExPASy Molecular Biology Server.
Number of amino acids: 220
Molecular weight: 25096.7
Theoretical pI: 6.16
Amino acid composition:
Ala (A) 12 5.5%
Arg (R) 12 5.5%
Asn (N) 13 5.9%
Asp (D) 11 5.0%
Cys (C) 5 2.3%
Gln (Q) 9 4.1%
Glu (E) 16 7.3%
Gly (G) 16 7.3%
His (H) 12 5.5%
Ile (I) 14 6.4%
Leu (L) 28 12.7%
Lys (K) 10 4.5%
Met (M) 5 2.3%
Phe (F) 5 2.3%
Pro (P) 6 2.7%
Ser (S) 10 4.5%
Thr (T) 9 4.1%
Trp (W) 6 2.7%
Tyr (Y) 5 2.3%
Val (V) 16 7.3%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 1117
Hydrogen H 1756
Nitrogen N 318
Oxygen O 321
Sulfur S 10
Formula: C1117H1756N318O321S10
Total number of atoms: 3522
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 39940 40854 40925 40780 39840
Abs 0.1% (=1 g/l) 1.591 1.628 1.631 1.625 1.587
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 39650 40600 40685 40540 39600
Abs 0.1% (=1 g/l) 1.580 1.618 1.621 1.615 1.578
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.94
This classifies the protein as stable.
Aliphatic index: 101.00
Grand average of hydropathicity (GRAVY): -0.231